Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants
| Autor: | Milena Marsoni, Marcella Bracale, Elena Baldoni, Michela Osnato, Monica Mattana, Enrico Magnani, I. Coraggio, Candida Vannini, Franca Locatelli |
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| Rok vydání: | 2004 |
| Předmět: |
abiotic stress
Arabidopsis thaliana Acclimatization Myb transcriptional factor abiotic stress cold tolerance Oryza sativa Arabidopsis thaliana transgenic plants Molecular Sequence Data Arabidopsis Oryza sativa Plant Science Genetically modified crops Biology transgenic plants Gene Expression Regulation Plant Sequence Homology Nucleic Acid Myb transcriptional factor Botany Genetics Promoter Regions Genetic Gene Plant Proteins Regulation of gene expression Base Sequence Abiotic stress fungi Plant physiology food and beverages Oryza Cell Biology cold tolerance biology.organism_classification Plants Genetically Modified Cell biology Cold Temperature Coleoptile Transcription Factors |
| Zdroj: | Plant journal 37 (2004): 115–127. doi:10.1046/j.1365-313X.2003.01938.x info:cnr-pdr/source/autori:Vannini C.; Locatelli F.; Bracale M.; Magnani E.; Marsoni M.; Osnato M.; Mattana M.; Baldoni E.; Coraggio I./titolo:Overexpression of the rice Osmyb4 gene increases chilling and freezing tolerance of Arabidopsis thaliana plants/doi:10.1046%2Fj.1365-313X.2003.01938.x/rivista:Plant journal (Print)/anno:2004/pagina_da:115/pagina_a:127/intervallo_pagine:115–127/volume:37 |
| Popis: | The expression of the gene Osmyb4, detected at low level in rice (Oryza sativa) coleoptiles grown for 3 days at 29 degrees C, is strongly induced by treatments at 4 degrees C. At sublethal temperatures of 10 and 15 degrees C, its expression in rice seedlings is already evident, but this effect cannot be vicariated by other stresses or ABA treatment. We demonstrate by transient expression that Myb4 transactivates the PAL2, ScD9 SAD and COR15a cold-inducible promoters. The Osmyb4 function in vivo is demonstrated overexpressing its cDNA in Arabidopsis thaliana plants (ecotype Wassilewskija) under the control of the constitutive CaMV 35S promoter. Myb4 overexpressing plants show a significant increased cold and freezing tolerance, measured as membrane or Photosystem II (PSII) stability and as whole plant tolerance. Finally, in Osmyb4 transgenic plants, the expression of genes participating in different cold-induced pathways is affected, suggesting that Myb4 represents a master switch in cold tolerance. |
| Databáze: | OpenAIRE |
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