A magainin-2 like bacteriocin BpSl14 with anticancer action from fish gut Bacillus safensis SDG14
| Autor: | Bindiya Ellathuparambil Saidumohamed, Tina Kollannoor Johny, Sarita G. Bhat, Unnikrishanan Babukuttan Sheela, Anitharaj Pottekkat Baburaj, Maya Sreeranganathan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Bacillus safensis
Lung Neoplasms Biophysics Antineoplastic Agents Apoptosis Bacillus Magainins 01 natural sciences Biochemistry 03 medical and health sciences chemistry.chemical_compound Bacteriocins Bacteriocin Peptide mass fingerprinting Tandem Mass Spectrometry Transforming Growth Factor beta Animals Humans Molecular Biology 030304 developmental biology 0303 health sciences biology Chemistry 010401 analytical chemistry Fishes Magainin Cell Biology biology.organism_classification Antimicrobial Protein tertiary structure 0104 chemical sciences Molecular Weight Receptors TNF-Related Apoptosis-Inducing Ligand A549 Cells Docking (molecular) Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Indolicidin |
| Zdroj: | Analytical Biochemistry. 627:114261 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/j.ab.2021.114261 |
| Popis: | Bacteriocins are gaining utmost importance in antimicrobial and chemotherapy due to their diverse structure and activity. This study centres on magainin-2 like bacteriocin with anticancer action, produced by Bacillus safensis strain SDG14 isolated from gut of marine fish Sardinella longiceps. The purified bacteriocin designated as BpSl14 was thermostable and pH tolerant. The molecular weight of BpS114 was estimated to be 6061.2 Da using MALDI-ToF MS. The partial primary sequence was elucidated by peptide mass fingerprinting using MALDI MS/MS. The tertiary structure of the partial sequence was similar to that of two magainin-2 α-helices joined together by extended indolicidin. The BpSl14 protein inhibited the cells of lung carcinoma, one of the deadliest cancers. Docking studies conducted with DR5 and TGF-β, two of the most prominent apoptotic receptors in adenocarcinoma, also proved the anti-apoptotic action of BpSl14. |
| Databáze: | OpenAIRE |
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