Stimulation of myeloid colony growth from peripheral blood by medium with an initially low osmolality
| Autor: | G. B. Tennant |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Colony-forming unit
Myeloid Osmotic concentration Osmolar Concentration digestive oral and skin physiology Humidity Stimulation Cell Biology Granulocyte Biology Stimulation Chemical Culture Media Hematopoiesis Colony-Forming Units Assay Andrology Incubators medicine.anatomical_structure Hypotonic Solutions Cell culture Culture Techniques Immunology medicine Humans Progenitor cell Incubation |
| Zdroj: | The International Journal of Cell Cloning. 8:123-129 |
| ISSN: | 0737-1454 |
| DOI: | 10.1002/stem.5530080205 |
| Popis: | The clonal growth of myeloid colonies from peripheral blood was maximal when cultures were established with an initial osmolality of 220 mosmol/kg which increased during incubation as a result of partial drying. When osmolality was stabilized by secondary humidification, the optimum osmolality was 270 mosmol/kg, but growth was always two- to fivefold less than similar cultures established at low osmolality and incubated on an open shelf. Cultures established at 270 mosmol/kg or above were statistically similar whether or not drying was eliminated. Maximum colonies were apparent after 14 days incubation under both conditions; addition of conditioned medium did not alter the pattern of growth. The greater sensitivity of cultures established at 220 mosmol/kg is advantageous when assaying circulating progenitors in pathological conditions where a low number of granulocyte/macrophage colony-forming units is common. |
| Databáze: | OpenAIRE |
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