Effects of Propofol on Several Membrane Characteristics of Cervical Cancer Cell Lines
| Autor: | Anqi Wu, Yanpeng Ci, Jinghe Wang, Enyou Li, Shuping Li, Fan Zhang, Wei Hou, Yuanfei Yao, Yinghua Cui, Changsong Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Physiology Cell Uterine Cervical Neoplasms Cell morphology lcsh:Physiology lcsh:Biochemistry HeLa Cell membrane 03 medical and health sciences 0302 clinical medicine Cell Movement Humans Medicine lcsh:QD415-436 Cytoskeleton Cell Shape Propofol Migration Cell Proliferation lcsh:QP1-981 biology business.industry Cell Membrane Cell migration biology.organism_classification Cell biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Ultrastructure Female AFM business Wound healing HeLa cell HeLa Cells |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 40, Iss 1-2, Pp 172-182 (2016) |
| ISSN: | 1421-9778 1015-8987 |
| DOI: | 10.1159/000452535 |
| Popis: | Background: Although significant advances have been made toward understanding the molecular mechanisms underlying the effect of propofol on tumor cell metastasis, less is known regarding how cell membrane and cytoskeletal ultrastructure are affected in this process. Here, we investigated the relationship between cell morphology and cell size, which are features mainly defined by the cytoskeleton. Methods: To confirm the effects of propofol on the migratory ability of human cervical carcinoma cells, cell migration and invasion were examined through scratch wound healing and transwell membrane assays. Furthermore, HeLa cells cultivated with different concentrations of propofol were examined by confocal microscopy and atomic force microscopy (AFM), and the mean optical density and migration ability of these cells were also assessed. In addition, cell membrane morphology was inspected using AFM. Results: The results of the wound healing and transwell membrane assays indicated that propofol decreases the migratory ability of cervical carcinoma cells compared to control cells. A comparative analysis of the test results revealed that short-term (3 h) exposure to propofol induced marked changes in cell membrane microstructure and in the cytoskeleton in a dose-dependent manner. These morphological changes in the cell membrane were accompanied by cytoskeleton (F-actin) derangement. The present findings demonstrate a close relationship between changes in cell membrane ultrastructure and cytoskeletal alterations (F-actin) in propofol-treated HeLa cells. AFM scanning analysis showed that cell membrane ultrastructure was significantly changed, including a clear reduction in membrane roughness. Conclusion: The influence of propofol on the HeLa cell cytoskeleton can be directly reflected by changes in cellular morphology, as assessed by AFM. Moreover, the use of AFM is a good method for investigating propofol-mediated changes within cytoskeletal ultrastructure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|