Antigenic characterization of Brazilian isolates of Anaplasma marginale
| Autor: | Midori Miguita, Cláudio R. Madruga, Jaqueline Bianque de Oliveira, Flábio R. Araújo, Maria A. M. Schenk, Raul H. Kessler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Microbiology (medical)
Anaplasmosis lcsh:Arctic medicine. Tropical medicine Anaplasma medicine.drug_class lcsh:RC955-962 Blotting Western lcsh:QR1-502 Cattle Diseases Monoclonal antibody complex mixtures Epitope lcsh:Microbiology Western blot Antigen parasitic diseases medicine Animals Fluorescent Antibody Technique Indirect B cell major surface protein Antigens Bacterial biology medicine.diagnostic_test Antibodies Monoclonal biology.organism_classification Virology Antigenic Variation Blot Anaplasma marginale Rickettsia medicine.anatomical_structure antigenic characterization Antigens Surface biology.protein Epitopes B-Lymphocyte Cattle Electrophoresis Polyacrylamide Gel Antibody Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 98, Issue: 3, Pages: 395-400, Published: APR 2003 Memórias do Instituto Oswaldo Cruz., Vol 98, Iss 3, Pp 395-400 (2003) |
| Popis: | Antigenic characterization of Anaplasma marginale isolates, by identifying conserved and variable epitopes of major surface proteins (MSP), is an important tool for vaccine development against this rickettsia. The B cell epitopes of A. marginale isolates from three microregions of the State of Pernambuco and one from the State of Mato Grosso do Sul, Brazil, were characterized by indirect fluorescent antibody technique (IFAT) and Western blot (WB) with 15 monoclonal antibodies (MAbs). The epitope recognized by MAb ANA22B1 (MSP-1a) was conserved by IFAT and WB (73-81 kDa). MSP-2 epitopes recognized by MAbs ANAO58A2 and ANAO70A2 were conserved by IFAT, while ANAO50A2 and ANA66A2 epitopes were polymorphic; in the WB, the MAbs ANAO50A2 and ANAO70A2 identified bands of 45 kDa only in the Pernambuco-Mata isolate. None of the isolates reacted with MAb ANAR75C2 (MSP-3). The MSP-4 epitope recognized by MAb ANAR76A1 was conserved by IFAT, as well as the MSP-5 epitope recognized by MAb ANAF16C1 by IFAT and WB (16 kDa). The MAbs ANAR17A6, ANAR83B3, ANAR94C1, ANAO24D5 and ANAR19A6 identified conserved epitopes by IFAT. MSP-1, MSP-2 and MSP-4, which previously showed partial protection in experimental trials, are also potential immunogens to be employed in Brazil, due to the B cell epitope conservation. |
| Databáze: | OpenAIRE |
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