Destinations of Some Fast-Transported Proteins in Sensory Neurons of Bullfrog Sciatic Nerve
| Autor: | David L. Wilson, Rulli Rd |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Rana catesbeiana
Biological Transport Nerve Tissue Proteins Sensory system Anatomy Biology Sulfur Radioisotopes Sciatic Nerve Biochemistry Axons Sensory neuron Cellular and Molecular Neuroscience medicine.anatomical_structure Dorsal root ganglion Bullfrog Axoplasmic transport medicine Biophysics Animals Electrophoresis Polyacrylamide Gel Neurons Afferent Neuron Sciatic nerve Axon |
| Zdroj: | Journal of Neurochemistry. 48:134-140 |
| ISSN: | 1471-4159 0022-3042 |
| DOI: | 10.1111/j.1471-4159.1987.tb13137.x |
| Popis: | Many characteristics of proteins that are fast axonally transported have been described, but the destinations of most within the neuron remain unknown. We have studied the destinations of some fast-transported proteins in sensory neurons of the bullfrog sciatic nerve, specifically to determine which may be deposited in axons and which may be destined for more distal, possibly terminal, areas. Dorsal root ganglia were pulse-labeled with [35S]methionine in vitro, following which they were separated from the sciatic nerve. After additional periods of transport, radioactive proteins from two areas of the nerve were separated by two-dimensional polyacrylamide gel electrophoresis and used to develop x-ray film. The first area contained the wavefront of transported radioactivity (wavefront region), whereas the second area was taken from nerve through which the wavefront had already passed (plateau region). The amount of radioactivity in certain fast-transported protein species from each area was determined by computer analysis of digitized video images of fluorographs. Certain proteins were preferentially left behind the wavefront and, therefore, may supply axon and possibly other nerve components, whereas other proteins were found almost exclusively in the wavefront and, hence, may supply more distal, possibly terminal, areas. |
| Databáze: | OpenAIRE |
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