Assessment Of Global And Gene-Specific Dna Methylation In Rat Liver And Kidney In Response To Non-Genotoxic Carcinogen Exposure
| Autor: | Osman Ugur Sezerman, Tao Chen, Angela Mally, Ilknur Melis Durasi, J. Kevin Chipman, Buket Alpertunga, Sibel Ozden, Neslihan Turgut Kara, Goksun Demirel |
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| Přispěvatelé: | Biruni Üniversitesi |
| Rok vydání: | 2015 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Time Factors Molecular Sequence Data Bisulfite sequencing Methapyrilene Biology Kidney Toxicology medicine.disease_cause Polymerase Chain Reaction Tandem Mass Spectrometry medicine Animals Methylated DNA immunoprecipitation Promoter Regions Genetic Rat Liver Chromatography High Pressure Liquid Oligonucleotide Array Sequence Analysis Pharmacology Non-Genotoxic Carcinogen Base Sequence Gene Expression Profiling MeDIP-Microarray Liver Neoplasms Methylation DNA Methylation Molecular biology Kidney Neoplasms Rats Inbred F344 Gene Expression Regulation Neoplastic Rat Kidney Cell Transformation Neoplastic Liver CpG site DNA methylation Carcinogens Cancer research CpG Islands Carcinogenesis medicine.drug DNA hypomethylation |
| Popis: | Altered expression of tumor suppressor genes and oncogenes, which is regulated in part at the level of DNA methylation, is an important event involved in non-genotoxic carcinogenesis. This may serve as a marker for early detection of non-genotoxic carcinogens. Therefore, we evaluated the effects of non-genotoxic hepatocarcinogens, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), hexachlorobenzene (HCB), methapyrilene (MPY) and male rat kidney carcinogens, d-limonene, p-dichlorobenzene (DCB), chloroform and ochratoxin A (OTA) on global and CpG island promoter methylation in their respective target tissues in rats. No significant dose-related effects on global DNA hypomethylation were observed in tissues of rats compared to vehicle controls using LC-MS/MS in response to short-term non-genotoxic carcinogen exposure. Initial experiments investigating gene-specific methylation using methylation-specific PCR and bisulfite sequencing, revealed partial methylation of p16 in the liver of rats treated with HCB and TCDD. However, no treatment related effects on the methylation status of Cx32, e-cadherin, VHL, c-myc, Igfbp2, and p15 were observed. We therefore applied genome-wide DNA methylation analysis using methylated DNA immunoprecipitation combined with microarrays to identify alterations in gene-specific methylation. Under the conditions of our study, some genes were differentially methylated in response to MPY and TCDD, whereas d-limonene, DCB and chloroform did not induce any methylation changes. 90-day OTA treatment revealed enrichment of several categories of genes important in protein kinase activity and mTOR cell signaling process which are related to OTA nephrocarcinogenicity. Deutscher Akademischer Austauschdienst Firat University Scientific Research Projects Management Unit: UDP-30102, NP-2919, UDP-17621, YADOP-20728 SBAG-109S187 |
| Databáze: | OpenAIRE |
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