Differences of cell growth and cell cycle regulators induced by basic fibroblast growth factor between nifedipine responders and non-responders
| Autor: | Yoshiaki Akimoto, Akihiko Gunji, Hidehiko Okada, Hiroko Matsumoto, Akira Fujii, Reiri Takeuchi, Mami Hori, Kosuke Hakozaki |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Cyclin E Nifedipine Antimetabolites Cyclin A Basic fibroblast growth factor Blotting Western Gingiva Cell Cycle Proteins Cell Line S Phase chemistry.chemical_compound Internal medicine medicine Humans Cell Proliferation Pharmacology biology Cell growth Cyclin-dependent kinase 2 lcsh:RM1-950 Cell Cycle G1 Phase DNA Cell cycle Calcium Channel Blockers Molecular biology Endocrinology lcsh:Therapeutics. Pharmacology chemistry Bromodeoxyuridine Cell culture biology.protein Molecular Medicine Fibroblast Growth Factor 2 |
| Zdroj: | Journal of Pharmacological Sciences, Vol 103, Iss 2, Pp 168-174 (2007) |
| ISSN: | 1347-8613 |
| Popis: | Differences of cell proliferation, cell cycle, and G1/S transition regulatory proteins of gingival fibroblasts derived from nifedipine-reactive patient (NIFr) and nifedipine-non-reactive patient (NIFn) in the presence of basic fibroblast growth factor (bFGF) were investigated to elucidate the mechanism of gingival overgrowth associated with nifedipine, one of the Ca2+-channel blockers. The proliferation rate of NIFr cells in the presence of bFGF significantly increased than NIFn cells. The proportion of NIFr cells that had undergone progression to the S and G2/M phases from the G0/G1 phase significantly increased compared to that in NIFn cells. Increases of pRB (Ser807/811), pCDK2 (Thr160), CDK2, and cyclin E protein levels in NIFr cells were greater than those in NIFn cells. The elevations of pRB (Ser780), RB, and cyclin A protein levels in NIFr cells did not differ from those of NIFn cells. The growth of NIFr cells was greater than that of NIFn cells as a result of the active G1/S transition of NIFr cells, as assessed by the increments of cyclin E, pCDK2, and pRB (ser807/811) protein in NIFr cells. Keywords:: gingival overgrowth, nifedipine, gingival fibroblast, cell cycle |
| Databáze: | OpenAIRE |
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