Centrosome–nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity
| Autor: | Jorge G Ferreira, Martial Balland, Vanessa Nunes, Matthieu Piel, Irène Wang, Domingos Castro, Margarida Dantas, Helder Maiato, Nicolas Carpi, Paulo Aguiar, Elisa Vitiello |
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| Přispěvatelé: | Instituto de Biologia Molecular e Celular - institute for molecular and cell biology [Porto, Portugal] (IBMC), Universidade do Porto, Laboratoire Interdisciplinaire de Physique [Saint Martin d’Hères] (LIPhy ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Instituto de Ciências Biomédicas de Abel Salazar (ICBAS) |
| Rok vydání: | 2020 |
| Předmět: |
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Cell division Nuclear Envelope Movement Mitosis Spindle Apparatus [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Prophase Actin-Related Protein 2-3 Complex 03 medical and health sciences 0302 clinical medicine Cell Adhesion medicine Molecular motor Humans Cytoskeleton Cell Shape Molecular Biology 030304 developmental biology Centrosome 0303 health sciences Cell Cycle Dyneins Articles Cell Biology Cell biology Spindle apparatus HEK293 Cells medicine.anatomical_structure Nucleus 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Molecular Biology of the Cell Molecular Biology of the Cell, American Society for Cell Biology, 2020, 31 (16), pp.1675-1690. ⟨10.1091/mbc.E20-01-0047⟩ |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | International audience; MBoC | ARTICLE Centrosome-nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity ABSTRACT During the initial stages of cell division, the cytoskeleton is extensively reorganized so that a bipolar mitotic spindle can be correctly assembled. This process occurs through the action of molecular motors, cytoskeletal networks, and the nucleus. How the combined activity of these different components is spatiotemporally regulated to ensure efficient spindle assembly remains unclear. To investigate how cell shape, cytoskeletal organization, and molecular motors cross-talk to regulate initial spindle assembly, we use a combination of micropatterning with high-resolution imaging and 3D cellular reconstruction. We show that during prophase, centrosomes and nucleus reorient so that centrosomes are positioned on the shortest nuclear axis at nuclear envelope (NE) breakdown. We also find that this orientation depends on a combination of centrosome movement controlled by Arp2/3-mediated regulation of microtubule dynamics and Dynein-generated forces on the NE that regulate nuclear reorientation. Finally, we observe this centrosome configuration favors the establishment of an initial bipolar spindle scaffold, facilitating chromosome capture and accurate segregation, without compromising division plane orientation. |
| Databáze: | OpenAIRE |
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