β-Amyloid Protein Structure Determines the Nature of Cytokine Release From Rat Microglia
| Autor: | Maj-Linda Bardyl Selenica, Anita Westlind-Danielsson, Marianne Schultzberg, Catharina Lindberg |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Protein Conformation medicine.drug_class medicine.medical_treatment Dose-Response Relationship Immunologic Peptide Biology Proinflammatory cytokine Cellular and Molecular Neuroscience Pregnancy Internal medicine medicine Amyloid precursor protein Animals Humans Secretion Rats Wistar Cells Cultured chemistry.chemical_classification Amyloid beta-Peptides Microglia General Medicine Receptor antagonist Peptide Fragments Rats Cell biology medicine.anatomical_structure Endocrinology Cytokine Animals Newborn chemistry biology.protein Cytokines Female Tumor necrosis factor alpha |
| Zdroj: | Journal of Molecular Neuroscience. 27:001-012 |
| ISSN: | 0895-8696 |
| Popis: | Activated microglia represent a major source of inflammatory factors in Alzheimer's disease and a possible source of cytotoxic factors. beta-Amyloid (Abeta) peptide, the predominant component in amyloid plaques, has been shown to activate microglia and stimulate their production of inflammatory factors. The present study was performed to analyze the responses of microglia to different forms of Abeta, with regard to release of the proinflammatory cytokines interleukin-1alpha (IL-1alpha), IL-1beta, tumor necrosis factor-alpha (TNF-alpha), IL-6, and interferon-gamma (IFN-gamma), as well as the IL-1 receptor antagonist (IL-1ra). Primary cultures of microglia from rat neonatal cerebral cortex were incubated with freshly dissolved Abeta1-40 or Abeta1-42, Abeta1-40 fibrils, Abeta1-40 betaamy balls, or vehicle. Abeta1-40 fibrils did not significantly stimulate any of these cytokines. Freshly dissolved Abeta1-40 resulted in a marked increase in the release of IL-1beta, and freshly dissolved Abeta1-42 significantly stimulated both IL-1alpha and IFN-gamma secretion. The Abeta1-40 betaamy balls stimulated the secretion of IL-1alpha and IL-1beta. Incubation with Abeta peptides did not affect the secretion of IL-1ra, IL-6, or TNF-alpha. In the case of IL-1beta, the response is correlated with the presence of Abeta peptide as monomers and oligomers. |
| Databáze: | OpenAIRE |
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