Pest control and resistance management through release of insects carrying a male-selecting transgene

Autor: Anthony M. Shelton, Thea Marubbi, Adam Walker, T.G. Emyr Davies, Nina Alphey, Luke Alphey, Neil I. Morrison, Kevin Gorman, Hilda L. Collins, Tim Harvey-Samuel, Yao Ju, Simon Warner
Jazyk: angličtina
Rok vydání: 2015
Předmět:
0106 biological sciences
Integrated pest management
Male
Physiology
Pest
Plant Science
Moths
01 natural sciences
Transgenic
Animals
Genetically Modified
Insecticide Resistance
Sterile insect technique
Structural Biology
Bacillus thuringiensis
Plutella xylostella
Transgenes
2. Zero hunger
0303 health sciences
education.field_of_study
Diamondback moth
Agricultural and Biological Sciences(all)
biology
food and beverages
Plants
Genetically Modified
Female
General Agricultural and Biological Sciences
Genetic Engineering
Biotechnology
Research Article
Crops
Agricultural
Population
Brassica
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
Animals
education
Pest Control
Biological
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Biochemistry
Genetics and Molecular Biology(all)
business.industry
fungi
Pest control
Cell Biology
biology.organism_classification
010602 entomology
Cry1Ac
PEST analysis
business
Insecticide resistance management
Insect
Developmental Biology
Zdroj: BMC Biology
ISSN: 1741-7007
Popis: Background Development and evaluation of new insect pest management tools is critical for overcoming over-reliance upon, and growing resistance to, synthetic, biological and plant-expressed insecticides. For transgenic crops expressing insecticidal proteins from the bacterium Bacillus thuringiensis (‘Bt crops’) emergence of resistance is slowed by maintaining a proportion of the crop as non-Bt varieties, which produce pest insects unselected for resistance. While this strategy has been largely successful, multiple cases of Bt resistance have now been reported. One new approach to pest management is the use of genetically engineered insects to suppress populations of their own species. Models suggest that released insects carrying male-selecting (MS) transgenes would be effective agents of direct, species-specific pest management by preventing survival of female progeny, and simultaneously provide an alternative insecticide resistance management strategy by introgression of susceptibility alleles into target populations. We developed a MS strain of the diamondback moth, Plutella xylostella, a serious global pest of crucifers. MS-strain larvae are reared as normal with dietary tetracycline, but, when reared without tetracycline or on host plants, only males will survive to adulthood. We used this strain in glasshouse-cages to study the effect of MS male P. xylostella releases on target pest population size and spread of Bt resistance in these populations. Results Introductions of MS-engineered P. xylostella males into wild-type populations led to rapid pest population decline, and then elimination. In separate experiments on broccoli plants, relatively low-level releases of MS males in combination with broccoli expressing Cry1Ac (Bt broccoli) suppressed population growth and delayed the spread of Bt resistance. Higher rates of MS male releases in the absence of Bt broccoli were also able to suppress P. xylostella populations, whereas either low-level MS male releases or Bt broccoli alone did not. Conclusions These results support theoretical modeling, indicating that MS-engineered insects can provide a powerful pest population suppressing effect, and could effectively augment current Bt resistance management strategies. We conclude that, subject to field confirmation, MS insects offer an effective and versatile control option against P. xylostella and potentially other pests, and may reduce reliance on and protect insecticide-based approaches, including Bt crops.
Databáze: OpenAIRE
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