Variations in genomic DNA methylation during the long-term in vitro proliferation of oil palm embryogenic suspension cultures
| Autor: | Esperanza Torres, Axel Labeyrie, James Tregear, Aline Personne, Tristan Durand-Gasselin, Alain Rival, Sylvie Doulbeau, Pascal Ilbert, Estelle Jaligot, Stéphane Dussert, Thierry Beulé |
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| Rok vydání: | 2013 |
| Předmět: |
Somatic embryogenesis
ADN Somaclonal variation Plant Science Arecaceae Elaeis guineensis F30 - Génétique et amélioration des plantes Epigenesis Genetic Variation somaclonale chemistry.chemical_compound Méthylation Embryogénèse somatique skin and connective tissue diseases Cells Cultured Chromatography High Pressure Liquid DNA methylation Micropropagation General Medicine Methylation Genomics Cell biology F02 - Multiplication végétative des plantes Seeds Plant Somatic Embryogenesis Techniques DNA Plant Biology Botany Génie génétique DNA Methylation biology.organism_classification genomic DNA chemistry Callus Epigenetic stability sense organs Stabilité génétique Agronomy and Crop Science DNA |
| Zdroj: | Plant Cell Reports |
| Popis: | KEY MESSAGE : The long-term proliferation of embryogenic cell suspensions of oil palm is associated with changes in both genomic methylation rates and embryogenic capacities. In the aim of exploring the relationship between epigenetic stability and the long-term in vitro proliferation of plant tissues, we have studied changes in genomic DNA methylation levels in embryogenic suspensions of oil palm (Elaeis guineensis Jacq.). Five embryogenic callus lines were obtained from selected hybrid seeds and then proliferated as suspension cultures. Each clonal line obtained from a single genotype was subdivided into three independent subclonal lines. Once established, cultures proliferated for 12 months and genomic DNA was sampled at 4 months intervals for the estimation of global DNA methylation rates through high performance liquid chromatography (HPLC) quantitation of deoxynucleosides. Our results show that in vitro proliferation induces DNA hypermethylation in a time-dependent fashion. Moreover, this trend is statistically significant in several clonal lines and shared between subclonal lines originating from the same genotype. Interestingly, the only clonal line undergoing loss of genomic methylation in the course of proliferation has been found unable to generate somatic embryos. We discuss the possible implications of genome-wide DNA methylation changes in proliferating cells with a view to the maintenance of genomic and epigenomic stability. |
| Databáze: | OpenAIRE |
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