Enantioselective capillary electrophoresis for pharmacokinetic analysis of methadone and 2‐ethylidene‐1,5‐dimethyl‐3,3‐diphenylpyrrolidine in equines anesthetized with ketamine and isoflurane
| Autor: | Friederike Andrea Sandbaumhüter, Regula Theurillat, M. Paula Larenza Menzies, Claudia Gittel, Wolfgang Thormann, Christina Braun |
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| Rok vydání: | 2019 |
| Předmět: |
Analyte
Pyrrolidines Metabolite Clinical Biochemistry 02 engineering and technology Sensitivity and Specificity 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Capillary electrophoresis Bolus (medicine) Pharmacokinetics medicine Animals Drug Interactions Horses Chromatography Isoflurane Chemistry 010401 analytical chemistry Electrophoresis Capillary Reproducibility of Results Stereoisomerism 021001 nanoscience & nanotechnology 0104 chemical sciences Ketamine Enantiomer 0210 nano-technology Methadone medicine.drug |
| Zdroj: | ELECTROPHORESIS. |
| ISSN: | 1522-2683 0173-0835 |
| Popis: | An enantioselective assay for the determination of methadone and its main metabolite 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in equine plasma based on capillary electrophoresis with highly sulfated γ-cyclodextrin as chiral selector and electrokinetic analyte injection is described. The assay is based on liquid/liquid extraction of the analytes at alkaline pH from 0.1 mL plasma followed by electrokinetic sample injection of the analytes from the extract across a buffer plug without chiral selector. Separation occurs cationically at normal polarity in a pH 3 phosphate buffer containing 0.16% (w/v) of highly sulfated γ-cyclodextrin. The developed assay is precise (intra- and interday RSD < 4% and < 7%, respectively), is capable to determine enantiomer levels of methadone and 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine in plasma down to 2.5 ng/mL, and was successfully applied to monitor enantiomer drug and metabolite levels in plasma of a pony that was anesthetized with racemic ketamine and isoflurane and received a bolus of racemic methadone and a bolus followed by constant rate infusion of racemic methadone. The data suggest that the assay is well suited for pharmacokinetic purposes. |
| Databáze: | OpenAIRE |
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