Comprehensive analysis of AHL gene family and their expression under drought stress and ABA treatment in Populus trichocarpa
| Autor: | Chenghao Li, Hanzeng Wang, Mengqiu Zhang, Jia Yang, Xue Leng, Minzhen Zeng, Xuemei Xu, Fude Wang |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Populus trichocarpa Bioinformatics Drought tolerance Protein domain lcsh:Medicine AT-hook motif containing nuclear localized gene family Plant Science 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Negative selection Bioinformatic analysis Gene family Molecular Biology Gene Transcription factor 030304 developmental biology Genetics 0303 health sciences biology General Neuroscience lcsh:R fungi food and beverages Forestry qRT-PCR General Medicine biology.organism_classification Histone biology.protein General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | PeerJ PeerJ, Vol 9, p e10932 (2021) |
| ISSN: | 2167-8359 |
| DOI: | 10.7717/peerj.10932 |
| Popis: | The AT-hook motif nuclear-localized (AHL) family is a plant transcription factor family, which plays an important role in growth and development and stress responses. We identified and analyzed 37 AHL genes in poplar (Populus trichocarpa). Phylogenetic analysis classified the PtrAHL members into three subfamilies based on their conserved domain. All PtrAHL paralogous pairs evolved under purifying selection. The promoter analysis revealed the presence of stress-related and phytohormone-related cis-elements of the PtrAHL genes. Our analysis of the tissue-specific expression pattern of PtrAHL genes indicated their significance in tissue and organ development. Network-based prediction suggested that PtrAHL genes may interact with histone deacetylases (HDAC) and participate in the development of organs, such as roots. Drought negatively impacts plant growth and development. ABA is produced under osmotic stress condition, and it takes an important part in the stress response and tolerance of plants. Real-time quantitative PCR (qRT-PCR) showed that PtrAHL genes were induced by drought stress and ABA treatment. These insights into the expression of PtrAHL genes under stress provide a basis for PtrAHL gene functional analysis. Our study will help develop new breeding strategies to improve drought tolerance in poplar. |
| Databáze: | OpenAIRE |
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