Enhancer of zeste homolog 2 overexpression has a role in the development of anaplastic thyroid carcinomas

Autor: Giancarlo Troncone, Nadine Hornig, Eleonora Borbone, Zuzana Jasencakova, Angelo Ferraro, Valerio Orlando, Lovorka Stojic, Alfredo Fusco, Francesco Esposito
Přispěvatelé: Borbone, E., Troncone, Giancarlo, Ferraro, A., Jasencakova, Z., Stojic, L., Esposito, F., Hornig, N., Fusco, Alfredo, Orlando, V.
Rok vydání: 2011
Předmět:
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
POLYCOMB GROUP PROTEINS
Thyroid Carcinoma
Anaplastic
Biochemistry
Mice
Endocrinology
Paired Box Transcription Factors
TRANSGENIC MICE
Thyroid
EZH2
PROLIFERATION
Polycomb Repressive Complex 2
PROSTATE-CANCER
Up-Regulation
DNA-Binding Proteins
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Cell Transformation
Neoplastic
Disease Progression
EXPRESSION
medicine.medical_specialty
Mice
Transgenic
macromolecular substances
Biology
Thyroid carcinoma
PAX8 Transcription Factor
Internal medicine
Cell Line
Tumor
medicine
Cell Adhesion
Gene silencing
Animals
Humans
Enhancer of Zeste Homolog 2 Protein
Gene Silencing
Thyroid Neoplasms
CANCER-CELLS
Cell adhesion
Transcription factor
HISTONE H3
Cell Proliferation
Cell growth
Biochemistry (medical)
Molecular biology
GENE
Cell culture
METHYLTRANSFERASE
HeLa Cells
Transcription Factors
Zdroj: 96 (2011).
info:cnr-pdr/source/autori:Borbone E, Troncone G, Ferraro A, Jasencakova Z, Stojic L, Esposito F, Hornig N, Fusco A, Orlando V./titolo:Enhancer of zeste homolog 2 overexpression has a role in the development of anaplastic thyroid carcinomas./doi:/rivista:/anno:2011/pagina_da:/pagina_a:/intervallo_pagine:/volume:96
ISSN: 1945-7197
Popis: Context: Enhancer of zeste homolog 2 (EZH2) is a histone lysine methyltransferase belonging to the polycomb group protein family. Overexpression of EZH2 has been found in several human malignancies including hematological and solid tumors. Objectives: In this study we investigated the expression levels of EZH2 and its polycomb group protein partners in thyroid carcinoma tissues with different degrees of malignancy to identify potential new therapeutic targets for anaplastic thyroid carcinoma (ATC). Results: We show that high EZH2 expression levels are characteristic of undifferentiated ATC, whereas no significant changes were observed in well-differentiated papillary and follicular thyroid carcinomas as compared with normal thyroid. Knockdown of EZH2 in ATC cell lines results in cell growth inhibition, loss of anchorage-independent growth, migration, and invasion properties. Moreover, we demonstrate that EZH2 directly controls differentiation of ATC cells by silencing the thyroid specific transcription factor paired-box gene 8 (PAX8). Conclusions: EZH2 is specifically overexpressed in ATC, and it directly contributes to transcriptional silencing of PAX8 gene and ATC differentiation.
Databáze: OpenAIRE
Externí odkaz: