Factors affecting islet graft embolization in the liver of diabetic mice
| Autor: | Naoki Sakata, Richard E. Chinnock, Andre Obenaus, John W. Mace, Eba Hathout, Nathaniel K. Chan |
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| Rok vydání: | 2009 |
| Předmět: |
endocrine system
Pathology medicine.medical_specialty Necrosis endocrine system diseases Endocrinology Diabetes and Metabolism medicine.medical_treatment Islets of Langerhans Transplantation H&E stain Hepatic Veins Streptozocin Article Diabetes Mellitus Experimental Mice Endocrinology Risk Factors Animals Medicine Treatment Failure Embolization Venous Thrombosis Mice Inbred BALB C geography geography.geographical_feature_category TUNEL assay Portal Vein business.industry Insulin Graft Survival Islet Embolization Therapeutic Transplantation Liver Apoptosis Female medicine.symptom business |
| Zdroj: | Islets. 1:26-33 |
| ISSN: | 1938-2022 1938-2014 |
| Popis: | BACKGROUND: Embolic occlusion of the portal vein due to islet transplantation is one of the major reasons for reduced survival of transplanted islets. In this study, we examined the location of islets as well as the correlation between islet and portal vein size after intraportal islet transplantation and evaluated liver and islet pathology. RESULTS: The liver was divided into peripheral and central sites. Islet and liver apoptosis/necrosis were significantly higher at peripheral sites. In regions without liver apoptosis or necrosis, portal vein diameter was significantly larger and embolic ratios were significantly lower. METHODS: BALB/c mice were intraportally transplanted with 800 islets and the liver was examined at postoperative day (POD) 0 (n = 7), POD 2 (n = 4) and POD 28 (n = 3). Liver specimens were stained for hematoxylin and eosin (necrosis), insulin and TUNEL (apoptosis). We evaluated distance from liver surface to islets, islet and portal vein diameter, embolic ratio (islet diameter/portal vein diameter), apoptosis/necrosis of islets and apoptosis/necrosis of the liver tissue surrounding the islet. CONCLUSION: Transplanted islets and liver tissue exhibited more injury at peripheral sites, in part, due to smaller diameters of portal venules that result in more frequent emboli following islet transplantation. |
| Databáze: | OpenAIRE |
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