Co-option of the PRDM14–CBFA2T complex from motor neurons to pluripotent cells during vertebrate evolution

Autor: Jr-Kai Yu, Shota Hashimoto, Yoshiaki Suwa, Daisuke Kawamura, Yoshiyuki Seki, Che-Yi Lin, Masanori Kawaguchi, Koji Takino, Shota Higashida, Kazumi Matsubara, Luok Wen Yong, Kota Sugiyama, Shigehiro Kuraku
Rok vydání: 2019
Předmět:
Pluripotent Stem Cells
Embryo
Nonmammalian
animal structures
Gene regulatory network
Synteny
Mice
03 medical and health sciences
0302 clinical medicine
Protein Domains
Sequence Homology
Nucleic Acid
biology.animal
medicine
Animals
Amino Acid Sequence
Molecular Biology
Zebrafish
Sea urchin
Transcription factor
Phylogeny
Lancelets
030304 developmental biology
Mice
Knockout
Motor Neurons
0303 health sciences
biology
Gene Expression Regulation
Developmental
RNA-Binding Proteins
Mouse Embryonic Stem Cells
Embryo
DNA Methylation
Motor neuron
biology.organism_classification
Biological Evolution
Embryonic stem cell
Cell biology
DNA Demethylation
DNA-Binding Proteins
Repressor Proteins
medicine.anatomical_structure
Sea Urchins
Vertebrates
embryonic structures
Biomarkers
030217 neurology & neurosurgery
Function (biology)
Protein Binding
Transcription Factors
Developmental Biology
Zdroj: Development.
ISSN: 1477-9129
0950-1991
Popis: Gene regulatory networks underlying cellular pluripotency are controlled by a core circuitry of transcription factors in mammals, including POU5F1. However, the evolutionary origin and transformation of pluripotency-related transcriptional networks have not been elucidated in deuterostomes. PR domain-containing protein 14 (PRDM14) is specifically expressed in pluripotent cells and germ cells, and is required for establishing embryonic stem cells (ESCs) and primordial germ cells in mice. Here, we compared the functions and expression patterns of PRDM14 orthologues within deuterostomes. Amphioxus PRDM14 and zebrafish PRDM14, but not sea urchin PRDM14, compensated for mouse PRDM14 function in maintaining mouse ESC pluripotency. Interestingly, sea urchin PRDM14 together with sea urchin CBFA2T, an essential partner of PRDM14 in mouse ESCs, complemented the self-renewal defect in mouse Prdm14 KO ESCs. Contrary to the Prdm14 expression pattern in mouse embryos, Prdm14 was expressed in motor neurons of amphioxus embryos, as observed in zebrafish embryos. Thus, Prdm14 expression in motor neurons was conserved in non-tetrapod deuterostomes and the co-option of the PRDM14-CBFA2T complex from motor neurons into pluripotent cells may have maintained the transcriptional network for pluripotency during vertebrate evolution. This article has an associated ‘The people behind the papers’ interview.
Databáze: OpenAIRE
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