Molecular characterization of dihydroneopterin aldolase and aminodeoxychorismate synthase in common bean—genes coding for enzymes in the folate synthesis pathway
| Autor: | Gregory E. Perry, Weilong Xie, Alireza Navabi, C. Joe Martin, Youn-Seb ShimY.S. Shim, K. Peter Pauls |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genotype Aminodeoxychorismate synthase Dihydroneopterin aldolase 01 natural sciences Protein Structure Secondary 03 medical and health sciences Folic Acid Genetics Molecular Biology Gene Transaminases Aldehyde-Lyases Plant Proteins Phaseolus chemistry.chemical_classification biology Chromosome Mapping food and beverages Sequence Analysis DNA General Medicine biology.organism_classification 030104 developmental biology Enzyme Biochemistry chemistry Multigene Family biology.protein Signal Transduction 010606 plant biology & botany Biotechnology |
| Zdroj: | Genome. 60:588-600 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/gen-2016-0227 |
| Popis: | Common beans (Phaseolus vulgaris) are excellent sources of dietary folates, but different varieties contain different amounts of these compounds. Genes coding for dihydroneopterin aldolase (DHNA) and aminodeoxychorismate synthase (ADCS) of the folate synthesis pathway were characterized by PCR amplification, BAC clone sequencing, and whole genome sequencing. All DHNA and ADCS genes in the Mesoamerican cultivar OAC Rex were isolated and compared with those genes in the genome of Andean genotype G19833. Both genotypes have two functional DHNA genes and one pseudo gene. PvDHNA1 and PvDHNA2 proteins have similar secondary structures and conserved residues as DHNA homologs in Staphylococcus aureus and Arabidopsis. Sequence analysis and synteny mapping indicated that PvDHNA1 might be a duplicated and transposed copy of PvDHNA2. There is only one ADCS gene (PvADCS) identified in the bean genome and it is identical in OAC Rex and G19833. PvADCS has the conserved motifs required for catalytic activity similar to other plant ADCS homologs. DHNA and ADCS gene-specific markers were developed, mapped, and compared to their physical locations on chromosomes 1 and 7, respectively. The gene-specific markers developed in this study should be useful for detection and selection of varieties with enhanced folate contents in bean breeding programs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|