Exogenous polyunsaturated fatty acids (PUFAs) promote changes in growth, phospholipid composition, membrane permeability and virulence phenotypes in Escherichia coli

Autor: Rachel N. Hofer, David K. Giles, Steven J. K. Symes, Timothy B. Simmons, Rachel E. Peters, Joshua L. Herndon
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Microbiology (medical)
Cell Membrane Permeability
Membrane permeability
Docosahexaenoic Acids
Movement
lcsh:QR1-502
Phospholipid
Biology
medicine.disease_cause
Microbiology
lcsh:Microbiology
03 medical and health sciences
chemistry.chemical_compound
Drug Resistance
Bacterial
medicine
Escherichia coli
Polyunsaturated fatty acids (PUFAs)
Beta oxidation
Phospholipids
030304 developmental biology
Polymyxin B
chemistry.chemical_classification
0303 health sciences
Arachidonic Acid
Virulence
030306 microbiology
Colistin
Biofilm
Cell Membrane
Fatty acid
Motility
biology.organism_classification
Anti-Bacterial Agents
Phenotype
chemistry
Biochemistry
Docosahexaenoic acid
Biofilms
Fatty Acids
Unsaturated
Antimicrobial peptides
Ampicillin
Bacteria
Polyunsaturated fatty acid
Research Article
Antimicrobial Cationic Peptides
Zdroj: BMC Microbiology
BMC Microbiology, Vol 20, Iss 1, Pp 1-12 (2020)
ISSN: 1471-2180
Popis: Background The utilization of exogenous fatty acids by Gram-negative bacteria has been linked to many cellular processes, including fatty acid oxidation for metabolic gain, assimilation into membrane phospholipids, and control of phenotypes associated with virulence. The expanded fatty acid handling capabilities have been demonstrated in several bacteria of medical importance; however, a survey of the polyunsaturated fatty acid responses in the model organism Escherichia coli has not been performed. The current study examined the impacts of exogenous fatty acids on E. coli. Results All PUFAs elicited higher overall growth, with several fatty acids supporting growth as sole carbon sources. Most PUFAs were incorporated into membrane phospholipids as determined by Ultra performance liquid chromatography-mass spectrometry, whereas membrane permeability was variably affected as measured by two separate dye uptake assays. Biofilm formation, swimming motility and antimicrobial peptide resistance were altered in the presence of PUFAs, with arachidonic and docosahexaenoic acids eliciting strong alteration to these phenotypes. Conclusions The findings herein add E. coli to the growing list of Gram-negative bacteria with broader capabilities for utilizing and responding to exogenous fatty acids. Understanding bacterial responses to PUFAs may lead to microbial behavioral control regimens for disease prevention.
Databáze: OpenAIRE
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