Overexpression of helper T cell type 2-related molecules in the skin of patients with eosinophilic dermatosis of hematologic malignancy
| Autor: | Francesco De Logu, Pamela Nardiello, Romina Nassini, Emiliano Antiga, Roberto Maglie, Elena Del Bianco, Gianna Baroni, Elisa Pasqualini, Filippo Ugolini, Daniela Massi, Sara Simi |
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| Rok vydání: | 2022 |
| Předmět: |
Chemokine CCL11
Pathology medicine.medical_specialty T cell Eosinophilic dermatosis Dermatology Th2 Cells Pemphigoid Bullous medicine Humans Interleukin 4 Retrospective Studies Cluster of differentiation business.industry Interleukins Forkhead Transcription Factors T-Lymphocytes Helper-Inducer medicine.disease medicine.anatomical_structure Interleukin 31 Hematologic Neoplasms Interleukin-4 Bullous pemphigoid CD5 business CD8 |
| Zdroj: | Journal of the American Academy of Dermatology. 87:761-770 |
| ISSN: | 0190-9622 |
| DOI: | 10.1016/j.jaad.2021.07.007 |
| Popis: | Background Eosinophilic dermatosis of hematologic malignancy (EDHM) is a rare dermatosis associated with blood tumors. Objective To characterize the expression of T-cell and B-cell markers and pruritogenic mediators in EDHM skin. Methods Immunohistochemical and immunofluorescence analysis were performed in 12 skin samples of EDHM, 11 samples of bullous pemphigoid (BP), and 5 samples from healthy controls (HC). Serum levels of interleukin (IL) 4 were analyzed in 11 patients with EDHM, 11 BP patients, and 5 HC by enzyme-linked immunosorbent assay. Results T-cell markers, including clusters of differentiation (CD) 3, CD4, CD8, and CD5 were significantly overexpressed in EDHM and BP skin compared to HC. A predominance of CD4+ over CD8+ cells and GATA3+ (helper T cell type 2 [Th2] marker) over T-bet+ (Th1 marker) cells were observed. FOXP3 expression was increased but the FOXP3/CD4 ratio was low. B-cell markers were under-represented, without significant differences between the 3 groups. IL-4 and IL-31 were significantly overexpressed in EDHM and BP compared to HC and colocalized with the Th2-associated marker GATA3. Eotaxin-1 was significantly overexpressed in EDHM compared to BP and HC. IL-4 serum concentration was significantly increased in EDHM and BP compared to HC. Limitations Small sample size; retrospective design. Conclusions Targeting Th2-related molecules, in particular IL-4, holds promise for EDHM management. |
| Databáze: | OpenAIRE |
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