Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study
| Autor: | Paula Zuñiga Saavedra, Eduardo Fernández Godoy, Gustavo Moncada Cortés, Osmir Batista de Oliveira Júnior, Patricio Vildósola Grez, Patricia Palma Fluxá |
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| Přispěvatelé: | University of Chile Operative Dentistry, Dental School Department of Restorative Dentistry, University of Chile Microbiology Dental School Department of Medicine and Oral Pathology, Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
food.ingredient
medicine.medical_treatment Dental restoration Dentistry Bacitracin engineering.material Clinical study Streptococcus mutans food Medicine Agar General Dentistry Toothpick Orthodontics biology dental restoration business.industry RK1-715 biology.organism_classification Amalgam (dentistry) stomatognathic diseases Tray engineering dental caries Dental caries business medicine.drug |
| Zdroj: | Brazilian Journal of Oral Sciences, Vol 12, Iss 4 (2015) SciELO Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Brazilian Journal of Oral Sciences v.12 n.4 2013 Brazilian journal of oral sciences Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP Brazilian Journal of Oral Sciences, Volume: 12, Issue: 4, Pages: 292-297, Published: DEC 2013 |
| ISSN: | 1677-3225 |
| Popis: | Made available in DSpace on 2015-08-06T16:14:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-12-01. Added 1 bitstream(s) on 2015-08-06T16:44:20Z : No. of bitstreams: 1 S1677-32252013000400004.pdf: 828129 bytes, checksum: 8440a74c0e44640d3dcaf74ae6e2e357 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) AIM: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. METHODS: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner' Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. RESULTS: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). CONCLUSIONS: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group. University of Chile Operative Dentistry, Dental School Department of Restorative Dentistry University of Chile Microbiology Dental School Department of Medicine and Oral Pathology Univ Estadual Paulista Araraquara Dental School Department of Restorative Dentistry Univ Estadual Paulista Araraquara Dental School Department of Restorative Dentistry |
| Databáze: | OpenAIRE |
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