Characterization of the Biological Role of Murine Erythroleukemia Cells 'Differentiation-Enhancing Factor' Using Antisense Oligodeoxynucleotides
| Autor: | Mauro Patrone, R. Falchetto, E. Melloni, Sandro Pontremoli, Mario Passalacqua, A. Pessino, B. Sparatore |
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| Rok vydání: | 1993 |
| Předmět: |
Ratón
Cellular differentiation Molecular Sequence Data Cell Drug Resistance Biophysics Peptide Biology Biochemistry Mice hemic and lymphatic diseases Tumor Cells Cultured medicine Animals Amino Acid Sequence HMGB1 Protein Molecular Biology Peptide sequence Genetics chemistry.chemical_classification Leukemia Experimental Base Sequence Antisense oligodeoxynucleotides Cell Differentiation Biological activity Cell Biology Oligonucleotides Antisense Neoplasm Proteins Cell biology medicine.anatomical_structure chemistry Vincristine Cell culture Leukemia Erythroblastic Acute |
| Zdroj: | Biochemical and Biophysical Research Communications. 193:941-947 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1993.1716 |
| Popis: | On the basis of the amino acid sequence of isolated tryptic peptides, it has been established that the differentiation enhancing factor, produced and active on murine erythroleukemia (MEL)cells, possesses a unique sequence, with no similarity to that of known proteins. Accordingly, this factor can be defined as a novel biologically active peptide. An antisense oligodeoxynucleotide, deduced from the sequence of a nonadecapeptide (produced by tryptic digestion of the factor), decreases the rate and the extent of MEL cell differentiation, induced by hexamethylenebisacetamide. In these cells the amount of the factor is reduced to one third of that constitutively present in untreated cells. Exogenous addition of the factor restores cell inducibility to normal values. Taken together, these results demonstrate the presence in MEL cells of a new factor, structurally and firnctionally unrelated to any of the known biologically active peptides, and suggest its crucial role in the promotion of an initial signal, in chemically induced erythroid differentiation. |
| Databáze: | OpenAIRE |
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