Effects of Zinc, Mercury, or Lead on [3H]MK-801 and [3H]Fluorowillardiine Binding to Rat Synaptic Membranes
| Autor: | José G. Ortiz, M. M. Rubio-Dávila, I. Rivera-Delgado, E. A. De Cardona-Juliá, N. Berríos-Cartagena, M. M. Feliciano-Bonilla |
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| Rok vydání: | 2021 |
| Předmět: |
Synaptic Membranes
chemistry.chemical_element Zinc AMPA receptor Neurotransmission Biochemistry Article Cellular and Molecular Neuroscience chemistry.chemical_compound Animals Alanine Glutamate receptor Transporter Mercury General Medicine Rats Neuroprotective Agents Pyrimidines Lead chemistry Amino acid neurotransmitter Excitatory postsynaptic potential Biophysics NMDA receptor Dizocilpine Maleate |
| Zdroj: | Neurochem Res |
| ISSN: | 1573-6903 0364-3190 |
| Popis: | Glutamate (Glu) is considered the most important excitatory amino acid neurotransmitter in the mammalian Central Nervous System. Zinc (Zn) is co-released with Glu during synaptic transmission and interacts with Glutamate receptors and transporters. We performed binding experiments using [(3)H]MK-801 (NMDA), and [(3)H]Fluorowillardine (AMPA) as ligands to study Zn-Glutamate interactions in rat cortical synaptic membranes. We also examined the effects of mercury and lead on NMDA or AMPA receptors. Zinc at 1nM, significantly potentiates [(3)H]MK-801 binding. Lead inhibits [(3)H]MK-801 binding at micromolar concentrations. At millimolar concentrations, Hg also has a significant inhibitory effect. These effects are not reversed by Zn (1nM). Zinc displaces the [(3)H]FW binding curve to the right. Lead (nM) and Hg (μM) inhibit [(3)H]FW binding. At certain concentrations, Zn reverses the effects of these metals on [(3)H]FW binding. These specific interactions serve to clarify the role of Zn, Hg, and Pb in physiological and pathological conditions. |
| Databáze: | OpenAIRE |
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