Extending the Lipidome Coverage by Combining Different Mass Spectrometric Platforms: An Innovative Strategy to Answer Chemical Food Safety Issues
| Autor: | Gaud Dervilly, Estelle Martineau, David Balgoma, Bruno Le Bizec, Anne-Lise Royer, Jérémy Marchand, Yann Guitton, Patrick Giraudeau |
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| Přispěvatelé: | Laboratoire d'étude des Résidus et Contaminants dans les Aliments (LABERCA), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
LC-HRMS
Health (social science) Computer science TP1-1185 Plant Science Computational biology 01 natural sciences Health Professions (miscellaneous) Microbiology Article 03 medical and health sciences Lactosylceramide Lipidomics β-agonist [CHIM]Chemical Sciences Lipidyzer™ music 030304 developmental biology 0303 health sciences music.instrument business.industry Chemical technology 010401 analytical chemistry Shotgun lipidomics Lipidome Serum samples Food safety Mass spectrometric 0104 chemical sciences 3. Good health ractopamine lipidomics business serum Food Science |
| Zdroj: | Foods Foods, MDPI, 2021, 10 (6), pp.1218. ⟨10.3390/foods10061218⟩ Volume 10 Issue 6 Foods, Vol 10, Iss 1218, p 1218 (2021) |
| ISSN: | 2304-8158 |
| DOI: | 10.3390/foods10061218⟩ |
| Popis: | From a general public health perspective, a strategy combining non-targeted and targeted lipidomics MS-based approaches is proposed to identify disrupted patterns in serum lipidome upon growth promoter treatment in pigs. Evaluating the relative contributions of the platforms involved, the study aims at investigating the potential of innovative analytical approaches to highlight potential chemical food safety threats. Serum samples collected during an animal experiment involving control and treated pigs, whose food had been supplemented with ractopamine, were extracted and characterised using three MS strategies: Non-targeted RP LC-HRMS the targeted Lipidyzer™ platform (differential ion mobility associated with shotgun lipidomics) and a homemade LC-HRMS triglyceride platform. The strategy enabled highlighting specific lipid profile patterns involving various lipid classes, mainly in relation to cholesterol esters, sphingomyelins, lactosylceramide, phosphatidylcholines and triglycerides. Thanks to the combination of non-targeted and targeted MS approaches, various compartments of the pig serum lipidome could be explored, including commonly characterised lipids (Lipidyzer™), triglyceride isomers (Triglyceride platform) and unique lipid features (non-targeted LC-HRMS). Thanks to their respective characteristics, the complementarity of the three tools could be demonstrated for public health purposes, with enhanced coverage, level of characterization and applicability. |
| Databáze: | OpenAIRE |
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