Rapid Detection of Bifidobacterium bifidum in Feces Sample by Highly Sensitive Quartz Crystal Microbalance Immunosensor
| Autor: | Zezhen Wu, Xiaojun Huang, Danjie Chen, Guiping Li, Danchun Lian, Kaijian Hou, Jilin Li, Dan Zhu, Pingsen Zhao, Yongru Chen, Yu Lin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
quartz crystal microbalance sensor
ved/biology.organism_classification_rank.species 02 engineering and technology 010402 general chemistry medicine.disease_cause 01 natural sciences digestive system feces sample lcsh:Chemistry Lactobacillus acidophilus fluids and secretions Listeria monocytogenes medicine Escherichia coli Feces Original Research Detection limit immunosensor Bifidobacterium bifidum Chromatography medicine.diagnostic_test ved/biology Chemistry Au nanoparticle food and beverages General Chemistry Quartz crystal microbalance 021001 nanoscience & nanotechnology 0104 chemical sciences lcsh:QD1-999 Immunoassay 0210 nano-technology |
| Zdroj: | Frontiers in Chemistry, Vol 8 (2020) Frontiers in Chemistry |
| ISSN: | 2296-2646 |
| DOI: | 10.3389/fchem.2020.00548/full |
| Popis: | In this work, a quartz crystal microbalance (QCM) sensor has been fabricated using immunoassay for sensitive determination of Bifidobacterium bifidum. Au nanoparticle has been used for amplifying sandwich assays. The proposed immunosensor exhibited a linear detection range between 103 and 105 CFU/mL with a limit of detection of 2.1 × 102 CFU/mL. The proposed immunosensor exhibited good selectivity for B. bifidum sensing with low cross reactivity for other foodborne pathogens such as Lactobacillus acidophilus, Listeria monocytogenes, and Escherichia coli. In addition, the proposed immunosensor has been successfully used for B. bifidum detection in feces samples and food samples. The frequency decreases of 12, 17, and 10 Hz were observed from the milk samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli. The frequency decreases of 8, 15, and 7 Hz were observed from the feces samples consisting of the mixtures of L. acidophilus, L. monocytogenes, and E. coli. |
| Databáze: | OpenAIRE |
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