Transition from Amorphous Cyclosporin A Nanoparticles to Size-Reduced Stable Nanocrystals in a Poloxamer 407 Solution
| Autor: | Kenjirou Higashi, Keisuke Ueda, Ziqiao Chen, Kunikazu Moribe |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ostwald ripening
Materials science Magnetic Resonance Spectroscopy Pharmaceutical Science Nanoparticle Poloxamer Amorphous solid Solutions symbols.namesake Chemical engineering Nanocrystal Microscopy Electron Transmission Solubility Transmission electron microscopy Cyclosporin a Drug Discovery symbols Cyclosporine Molecular Medicine Particle Nanoparticles Particle size Particle Size |
| Zdroj: | Molecular pharmaceutics. 19(1) |
| ISSN: | 1543-8392 |
| Popis: | Amorphous drug nanoparticles usually exhibit low storage stability. A comprehensive understanding of the molecular states and physicochemical properties of the product is indispensable for designing stable formulations. In the present study, an amorphous cyclosporin A (CyA) nanosuspension with a mean particle size of approximately 370 nm was prepared by wet bead milling with poloxamer 407 (P407). Interestingly, the prepared amorphous CyA nanoparticles were transformed into uniform CyA nanocrystals with a reduced mean particle size of approximately 200 nm during storage at 25 °C. The CyA nanocrystals were stably maintained for at least 1 month. The particle morphologies and molecular structures of the CyA nanosuspensions before and after storage were thoroughly characterized by cryogenic transmission electron microscopy and magic-angle spinning nuclear magnetic resonance spectroscopy, respectively. They revealed that the freshly prepared amorphous CyA nanoparticles (∼370 nm) were secondary particles composed of aggregated primary particles with an estimated size of 50 nm. A portion of P407 was found to be entrapped at the gaps between the primary particles due to aggregation, while most of P407 was dissolved in the solution either adsorbing at the solid/liquid interface or forming polymeric micelles. The entrapped P407 is considered to play an important role in the destabilization of the amorphous CyA nanoparticles. The resultant CyA nanocrystals (∼200 nm) were uniform single crystals of Form 2 hydrate and showed corner-truncated bipyramidal features. Owing to the narrow particle size distribution of the CyA nanocrystals, the rate of Ostwald ripening was slow, giving long-term stability to the CyA nanocrystals. This study provides new insights into the destabilization mechanism of amorphous drug nanoparticles. |
| Databáze: | OpenAIRE |
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