Characterization of acute rat parvovirus infection by in situ hybridization
| Autor: | Frank X. Paturzo, Robert O. Jacoby, Abigail L. Smith, Elizabeth A. Johnson, D J Gaertner, Janet L. Brandsma |
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| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
Endothelium Viremia In situ hybridization Biology Virus Replication Peripheral blood mononuclear cell Parvoviridae Virus Parvoviridae Infections Rats Sprague-Dawley Pathogenesis Virology medicine Animals Lung In Situ Hybridization Parvovirus RNA Probes biology.organism_classification medicine.disease Rats Infectious Diseases medicine.anatomical_structure DNA Viral Spleen |
| Zdroj: | Virus Research. 28:1-18 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/0168-1702(93)90085-2 |
| Popis: | In situ hybridization and virus titration were used to characterize early stages of rat virus (RV) infection of rat pups after oronasal inoculation. Results suggest that virus enters through the lung and that early viremia leads rapidly to pantropic infection. Cells derived from all three germ layers were infected with RV, but those of endodermal and mesodermal origin were the predominant targets. Infection of vascular endothelium was widespread and was associated with hemorrhage and infarction in the brain. Convalescence from acute infection was accompanied by mononuclear cell infiltrates at sites containing RV DNA. Viral DNA was also detected in endothelium, fibroblasts and smooth muscle myofibers four weeks after inoculation. Further examination of these cells as potential sites of persistent infection is warranted. |
| Databáze: | OpenAIRE |
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