Comparison of antigenic and chemical similarities between vitamin B12-binders of human gastric juice and vitamin B12-binders extracted from the mucosa of chimpanzee (Pan troglodytes) stomach
| Autor: | O. Neal Miller, Hans J. Hansen, G.D. Frentz |
|---|---|
| Rok vydání: | 1967 |
| Předmět: |
Intrinsic Factor
Male Antimetabolites Biochemistry Genetics and Molecular Biology (miscellaneous) Antibodies Anemia Pernicious medicine Animals Humans Vitamin B12 Antigens pernicious anemia Binding Sites Gastric Juice Intrinsic factor biology Stomach Hominidae medicine.disease Cobalt Isotopes Vitamin B 12 Titer medicine.anatomical_structure Biochemistry Gastric Mucosa Spectrophotometry Fundus (uterus) Sephadex Chromatography Gel biology.protein Antibody Protein Binding |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Protein Structure. 147:162-168 |
| ISSN: | 0005-2795 |
| DOI: | 10.1016/0005-2795(67)90099-2 |
| Popis: | Normal human gastric juice and an extract of chimpanzee fundus were studied by chromatography on Sephadex G-100 columns. Two B12-binders were demonstrated in both human gastric juice and chimpanzee fundus extract, one which eluted with proteins in the mol. wt. range of 90 000, the second which eluted with proteins having a mol. wt. of approx. 50 000. The small B12-binder in both human gastric juice and chimpanzee fundus extract reacted with the two antibodies found in serum of pernicious anemia patients that neutralize human intrinsic factor. When chimpanzee fundus extract was used as a source of intrinsic factor to determine the titer of the antibody in pernicious anemia serum which neutralizes the B12 combining site of intrinsic factor, identical titers were obtained as when human gastric juice was used as the source of intrinsic factor. |
| Databáze: | OpenAIRE |
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