Comparative understanding of UTS2 and UTS2R genes for their involvement in type 2 diabetes mellitus

Autor: Nancy S. Magnuson, Zhihua Jiang, Jennifer J. Michal, Zeping Wang, David J. Tobey, Michael D. MacNeil
Rok vydání: 2008
Předmět:
Male
UTS2R
Applied Microbiology and Biotechnology
Receptors
G-Protein-Coupled
chemistry.chemical_compound
0302 clinical medicine
UTS2
0303 health sciences
education.field_of_study
Chromosome Mapping
3. Good health
Adipose Tissue
Body Composition
Female
Research Paper
medicine.medical_specialty
Urotensins
Population
Single-nucleotide polymorphism
Carbohydrate metabolism
Biology
Polymorphism
Single Nucleotide
Cell Line
03 medical and health sciences
Insulin resistance
Internal medicine
Type 2 diabetes mellitus
medicine
Animals
Humans
Muscle
Skeletal
education
Molecular Biology
Gene
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Fatty acid metabolism
Type 2 Diabetes Mellitus
Cell Biology
Lipid Metabolism
medicine.disease
Obesity
Endocrinology
Diabetes Mellitus
Type 2
chemistry
skeletal fat accumulation
Cattle
fatty acid composition
030217 neurology & neurosurgery
Developmental Biology
Zdroj: International Journal of Biological Sciences
ISSN: 1449-2288
DOI: 10.7150/ijbs.4.96
Popis: Several reports have shown that urotensin 2 (UTS2) and its receptor (UTS2R) are involved in glucose metabolism and insulin resistance, which lead to development of type 2 diabetes mellitus (T2DM) in humans. In the present study, we annotated both bovine UTS2 and UTS2R genes and identified 5 single nucleotide polymorphisms (SNPs) for the former gene and 14 mutations for the latter gene. Four mutations were genotyped on a Wagyu x Limousin reference population, including 6 F(1 )bulls, 113 F(1 )dams and ~250 F(2 )progeny. Among 12 phenotypes related to fat deposition and fatty acid composition, we observed that the UTS2 gene was significantly associated with the amount of skeletal saturated fatty acids, while its receptor (UTS2R) gene had significant effects on amounts of saturated and monounsaturated fatty acids, Delta(9) desaturase activity for converting 16:0 into 16:1, muscle fat (marbling) score and Longissimus Dorsi muscle area. However, in this population, these markers were not associated with subcutaneous fat depth or percent kidney, pelvic and heart fat. We also found that mutations in the promoter regions altered the promoter activities in both genes and coding SNPs might affect the mRNA stability in the UTS2R gene. Overall, our present study provides the first evidence that both UTS2 and UTS2R genes regulate skeletal muscle fat accumulation and fatty acid metabolism, thus indicating their potential pathological functions related to obesity and T2DM in humans.
Databáze: OpenAIRE
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