Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria
| Autor: | Maya Angelovska, Christina Stoykova, Mila Kaleva, Vesselin Kussovski, Hristo Najdenski, Sevda Naydenska, Iva Tsvetkova, Maya M. Zaharieva, Yana Ilieva, Lyudmila Dimitrova |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) antibiotic resistance Antibiotic resistance medicine.drug_class PCR methods Antibiotic sensitivity Phoenix M50 030106 microbiology Antibiotics Virulence RM1-950 Biology medicine.disease_cause Biochemistry Microbiology Article 03 medical and health sciences Plasmid swine faeces and lagoons Escherichia coli medicine Pharmacology (medical) General Pharmacology Toxicology and Pharmaceutics Feces biochemical phenomena metabolism and nutrition bacterial infections and mycoses 030104 developmental biology Infectious Diseases VTEC Biofilms Therapeutics. Pharmacology biofilms Swine faeces and lagoons |
| Zdroj: | Antibiotics, Vol 10, Iss 940, p 940 (2021) Antibiotics Volume 10 Issue 8 |
| ISSN: | 2079-6382 |
| Popis: | Antimicrobial resistance (AMR) is a worldwide health problem affecting humans, animals, and the environment within the framework of the “One Health” concept. The aim of our study was to evaluate the prevalence of pathogenic strains of the species Escherichia coli (E. coli), their AMR profile, and biofilm-forming potential. The isolated strains from three swine faeces and free lagoons (ISO 16654:2001/Amd 1:2017) were confirmed using Phoenix M50 and 16S rDNA PCR. The antibiotic sensitivity to 34 clinically applied antibiotics was determined by Phoenix M50 and the disc diffusion method, according to the protocols of the CLSI and EUCAST. We confirmed the presence of 16 E. coli isolates, of which 87.5% were multi-drug-resistant and 31.25% performed strong biofilms. The possibility for the carrying and transmission of antibiotic-resistance genes to quinolones (qnr), aminoglycosides (aac(3)), β-lactamase-producing plasmid genes ampC, and blaSHV/blaTEM was investigated. We confirmed the carrying of blaSHV/blaTEM in one and ampC in seven isolates. The strains were negative for the virulence genes (ETEC (LT, STa, and F4), EPEC (eae), and STEC/VTEC (stx and stx2all)). The results should contribute to the development of effective measures for limitation and control on the use of antibiotics, which is a key point in the WHO action plan. |
| Databáze: | OpenAIRE |
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