Mechanisms of Bacterial (Serratia marcescens) Attachment to, Migration along, and Killing of Fungal Hyphae
| Autor: | Nir Osherov, Tal Hover, Roberto E. Bruna, Boris Fichtman, Tal Maya, Yulia Mitiagin, Yana Shadkchan, Eleonora García-Véscovi, Sapir Ron, Amnon Harel, Hani Sandovsky, Nitzan Kijner, Robert M. Q. Shanks |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Gram-negative bacteria Hypha Otras Ciencias Biológicas 030106 microbiology Hyphae migration Applied Microbiology and Biotechnology Bacterial Adhesion Microbiology purl.org/becyt/ford/1 [https] Ciencias Biológicas 03 medical and health sciences Antibiosis Environmental Microbiology purl.org/becyt/ford/1.6 [https] Pest Control Biological Serratia marcescens Mycelium Microbial Viability Ecology biology Chitinases fungi Fungal-bacterial interaction Fungi Biofilm biology.organism_classification Bacterial adhesin adhesion Flagella Biofilms Fimbriae Bacterial Host-Pathogen Interactions Mutation Chitinase Mucorales biology.protein CIENCIAS NATURALES Y EXACTAS Rhizopus Bacteria Zygomycota Food Science Biotechnology |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.04070-15 |
| Popis: | We have found a remarkable capacity for the ubiquitous Gram-negative rod bacterium Serratia marcescens to migrate along and kill the mycelia of zygomycete molds. This migration was restricted to zygomycete molds and several basidiomycete species. No migration was seen on any molds of the phylum Ascomycota. S. marcescens migration did not require fungal viability or surrounding growth medium, as bacteria migrated along aerial hyphae as well. S. marcescens did not exhibit growth tropism toward zygomycete mycelium. Bacterial migration along hyphae proceeded only when the hyphae grew into the bacterial colony. S. marcescens cells initially migrated along the hyphae, forming attached microcolonies that grew and coalesced to generate a biofilm that covered and killed the mycelium. Flagellum-defective strains of S. marcescens were able to migrate along zygomycete hyphae, although they were significantly slower than the wild-type strain and were delayed in fungal killing. Bacterial attachment to the mycelium does not necessitate type 1 fimbrial adhesion, since mutants defective in this adhesin migrated equally well as or faster than the wild-type strain. Killing does not depend on the secretion of S. marcescens chitinases, as mutants in which all three chitinase genes were deleted retained wild-type killing abilities. A better understanding of the mechanisms by which S. marcescens binds to, spreads on, and kills fungal hyphae might serve as an excellent model system for such interactions in general; fungal killing could be employed in agricultural fungal biocontrol. Fil: Hover, Tal. Tel Aviv University; Israel Fil: Maya, Tal. Tel Aviv University; Israel Fil: Ron, Sapir. Tel Aviv University; Israel Fil: Sandovsky, Hani. Tel Aviv University; Israel Fil: Shadkchan, Yana. Tel Aviv University; Israel Fil: Kijner, Nitzan. Tel Aviv University; Israel Fil: Mitiagin, Yulia. Tel Aviv University; Israel Fil: Fichtman, Boris. Bar Ilan University; Israel Fil: Harel, Amnon. Bar Ilan University; Israel Fil: Shanks, Robert M. Q.. University of Pittsburgh Medical Center; Estados Unidos Fil: Bruna, Roberto Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Garcia Vescovi, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina Fil: Osherov, Nir. Tel Aviv University; Israel |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|