Transport of Cl- across the plasma membrane during pollen grain germination in tobacco
| Autor: | N. P. Matveyeva, D. S. Andreyuk, I. P. Yermakov |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Nicotiana tabacum
Germination Biology 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid Biochemistry Chloride Antiporters chemistry.chemical_compound Chloride Channels Furosemide Tobacco medicine Channel blocker Bumetanide Benzoic acid Chromatography Ion Transport Niflumic acid food and beverages Niflumic Acid General Medicine Isoxazoles biology.organism_classification chemistry DIDS Nitrobenzoates Barbiturates Biophysics Pollen medicine.drug |
| Zdroj: | Biochemistry. Biokhimiia. 68(11) |
| ISSN: | 0006-2979 |
| Popis: | The role of Cl- transport across the plasma membrane was studied in an early step of pollen grain germination in tobacco Nicotiana tabacum L. The Cl- channel blockers, 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) and niflumic acid, completely suppress the germination with IC(50) approximately 8 micro M. At this concentration NPPB reduces the rate of Cl- efflux out of pollen grain by 1.8-fold in the interval 5-12 min, and niflumic acid reduces the rate 1.2-fold. 4,4;-Diisothiocyanatostilbene-2,2;-disulfonic acid, a known inhibitor of Cl- channels and antiporters, completely suppresses germination as well (IC(50) = 240 micro M), but has no effect on the rate of Cl- efflux. Inhibitors of chloride co-transporters, such as furosemide, bumetanide, and bis(1,3-dibutylbarbituric acid)pentamethine oxonol, suppress the germination by less than 50%. This set of data suggests that NPPB-sensitive anion channels are involved in the activation of pollen grains in the early stage of germination. |
| Databáze: | OpenAIRE |
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