Enzymatic Synthesis of Ricinoleyl Hydroxamic Acid Based on Commercial Castor Oil, Cytotoxicity Properties and Application as a New Anticancer Agent
| Autor: | Thomas J. Webster, Bahram Saleh, Bahareh Nikpey, Siavash Jahangirian, Katayoon Kalantari, Hossein Jahangirian, Roshanak Rafiee-Moghaddam |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Castor Oil
ricinoleyl hydroxamic acid Cell Survival Biophysics biomedicine Pharmaceutical Science Bioengineering Antineoplastic Agents 02 engineering and technology Hydroxylamine 010402 general chemistry 01 natural sciences Catalysis Biomaterials chemistry.chemical_compound International Journal of Nanomedicine Cell Line Tumor Drug Discovery lipozyme and castor oil medicine Hexanes Humans Cytotoxicity Melanoma Original Research enzymatic reaction chemistry.chemical_classification Hydroxamic acid Chemistry Brain Neoplasms Organic Chemistry General Medicine Lipase Fibroblasts 021001 nanoscience & nanotechnology 0104 chemical sciences anticancer agent Hexane Solvent Enzyme Castor oil Solvents 0210 nano-technology Glioblastoma Nuclear chemistry medicine.drug |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Background New anticancer agents that rely on natural/healthy, not synthetic/toxic, components are very much needed. Methods Ricinoleyl hydroxamic acid (RHA) was synthesized from castor oil and hydroxylamine using Lipozyme TL IM as a catalyst. To optimize the conversion, the effects of the following parameters were investigated: type of organic solvent, period of reaction, amount of enzyme, the molar ratio of reactants and temperature. The highest conversion was obtained when the reaction was carried out under the following conditions: hexane as a solvent; reaction period of 48 hours; 120 mg of Lipozyme TL IM/3 mmol oil; HA-oil ratio of 19 mmol HA/3 mmol oil; and temperature of 40°C. The cytotoxicity of the synthesized RHA was assessed using human dermal fibroblasts (HDF), and its application towards fighting cancer was assessed using melanoma and glioblastoma cancer cells over a duration of 24 and 48 hours. Results RHA was successfully synthesized and it demonstrated strong anticancer activity against glioblastoma and melanoma cells at as low as a 1 µg/mL concentration while it did not demonstrate any toxicity against HDF cells. Conclusion This is the first report on the synthesis of RHA with great potential to be used as a new anticancer agent. Video abstract Point your SmartPhone at the code above. If you have a QR code reader the video abstract will appear. Or use: https://youtu.be/E6EczO-1IHo |
| Databáze: | OpenAIRE |
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