Oxygen tension modulates extracellular vesicles and its miRNA contents in bovine embryo culture medium
| Autor: | Gabriella Mamede Andrade, M. M. Bomfim, Maite del Collado, Felipe Perecin, Juliano Coelho da Silveira, Flávio Vieira Meirelles |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cell type chemistry.chemical_element Biology Oxygen BIOTECNOLOGIA DA REPRODUÇÃO Embryo Culture Techniques 03 medical and health sciences Extracellular Vesicles 0302 clinical medicine microRNA Genetics Animals Secretion 030219 obstetrics & reproductive medicine Embryogenesis Embryo Cell Biology Embryo Mammalian In vitro Oxygen tension Cell biology Culture Media MicroRNAs 030104 developmental biology chemistry Cattle Female Developmental Biology |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1098-2795 |
| Popis: | The biotechnology for in vitro embryo production is becoming increasingly popular, being applied to humans and domestic animals. Embryo development can be achieved with either 20% or 5% oxygen tension. The extracellular vesicles (EVs) are secreted by different cell types and carry bioactive materials. Our objective was to determine the secretion pattern and micro RNA (miRNA) contents of EVs released in the bovine embryo culture environment-embryo and cumulus cell monolayer-on Days 3 and 7 of in vitro culture under two different oxygen tensions: High (20%) and low (5%). The EVs were isolated from the medium and analyzed to determine size, concentration, and miRNA levels. EVs concentration in low oxygen tension increased on Day 3 and decreased on Day 7. Additionally, altered EV miRNAs derived from the embryo-cumulus culture medium were predicted to regulate survival and proliferation-related pathways on Days 3 and 7. Moreover, miR-210 levels decreased in EVs isolated from the culture medium under high oxygen tension suggesting that this miRNA can be used as a marker for normoxia since it is associated with low oxygen tension. In summary, this study provides knowledge of the oxygen tension effects on EVs release and content, and potentially, on cell-to-cell communication during in vitro bovine embryo production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text