Toll-like receptor 4 deficient mice do not develop remifentanil-induced mechanical hyperalgesia: An experimental randomised animal study
| Autor: | Rocío Bustamante, Ignacio A. Gómez de Segura, Delia Aguado |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Mice
129 Strain medicine.medical_treatment Remifentanil (+)-Naloxone Sevoflurane 03 medical and health sciences Mice Random Allocation 0302 clinical medicine 030202 anesthesiology Physical Stimulation medicine Animals Receptor Saline Mice Knockout business.industry Analgesics Opioid Mice Inbred C57BL Toll-Like Receptor 4 Anesthesiology and Pain Medicine Nociception Hyperalgesia Anesthesia TLR4 Female medicine.symptom business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | European journal of anaesthesiology. 35(7) |
| ISSN: | 1365-2346 |
| Popis: | BACKGROUND Drugs with antagonistic actions on the Toll-like receptor 4 (Tlr4), such as naloxone at ultra low doses, have been used to inhibit opioid-induced hyperalgesia in rodents suggesting the involvement of this receptor and pathway on opioid-induced hyperalgesia. OBJECTIVE The aim of this study was to determine whether mice without the Tlr4 gene (Tlr4) would not develop remifentanil-induced hyperalgesia. DESIGN An experimental randomised animal study. SETTING Experimental Unit, Complutense University of Madrid, Madrid, Spain. ANIMALS Twelve adult female wild-type mice and 12 adult Tlr4 mice. INTERVENTIONS Under sevoflurane anaesthesia, a 1-h, constant rate subcutaneous infusion of remifentanil (4 μg kg min) or 0.9% saline. MAIN OUTCOME MEASURES Mechanical nociceptive thresholds were evaluated using a von Frey hair test before (baseline) and on days 5, 6 and 7 after treatment. Hyperalgesia was considered to be a decrease in the mechanical nociceptive threshold. Changes in mechanical nociceptive thresholds in the different groups were compared with one-sided paired t tests. RESULTS Baseline mechanical nociceptive thresholds were similar in all groups (2.2 ± 0.1 g). Remifentanil produced a 24% decrease in mechanical nociceptive thresholds in the wild-type mice (1.7 ± 0.0 g, averaged over 3 days, P = 0.00021), whereas the nociceptive thresholds were not changed in Tlr4 mice (2.2 ± 0.1 g, P = 0.857) or in mice receiving 0.9% saline (Tlr4, 2.2 ± 0.1 g, P = 0.807; wild-type, 2.2 ± 0.1 g, P = 0.962). CONCLUSION Tlr4 receptor involvement is suggested in the development of remifentanil-induced hyperalgesia in mice. TRIAL REGISTRATION CEA-UCM 107/2012. |
| Databáze: | OpenAIRE |
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