FT-IR Analysis of P. aeruginosa Bacteria Inactivation by Femtosecond IR Laser Radiation

Autor:	Irina Saraeva, Eteri Tolordava, Svetlana Sheligyna, Alyona Nastulyavichus, Roman Khmelnitskii, Nikolay Pokryshkin, Dmitriy Khmelenin, Sergey Kudryashov, Andrey Ionin, Andrey Akhmatkhanov
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Inorganic Chemistry Fourier-transform IR spectroscopy IR laser inactivation bacteria Organic Chemistry General Medicine Physical and Theoretical Chemistry Molecular Biology Spectroscopy Catalysis Computer Science Applications
Zdroj:	International Journal of Molecular Sciences; Volume 24; Issue 6; Pages: 5119
ISSN:	1422-0067
DOI:	10.3390/ijms24065119
Popis:	We report the successful inactivation of P. aeruginosa strain by femtosecond infrared (IR) laser radiation at the resonant wavelengths of 3.15 μm and 6.04 μm, chosen due to the presence of characteristic molecular vibrations in the main structural elements of the bacterial cells in these spectral ranges: vibrations of amide groups in proteins (1500–1700 cm−1), and C-H vibrations in membrane proteins and lipids (2800–3000 cm−1). The underlying bactericidal structural molecular changes were revealed by the stationary Fourier-transform IR spectroscopy, with the spectral peaks parameters being obtained by Lorentzian fitting with the hidden peaks revealed by the second derivative calculations, while no visible damage to the cell membranes was identified by scanning and transmission electron microscopy.
Databáze:	OpenAIRE
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