Mutations in blind cavefish target the light-regulated circadian clock gene, period 2
| Autor: | Ceinos, R. M., Frigato, E., Pagano, C., Fr��hlich, N., Negrini, P., Cavallari, N., Vallone, D., Fuselli, S., Bertolucci, C., Foulkes, N. S. |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Life sciences
biology Fish Proteins extraretinal photoreception genetic structures Light Cyprinidae lcsh:Medicine 2411 Fisiología Humana Circadian clock Evolution Molecular ddc:570 Animals lcsh:Science Evolution of the circadian clock extraretinal photoreception cavefish Circadian clock Zebrafish 2409 Genética lcsh:R Ambientale Period Circadian Proteins Zebrafish Proteins 2401.13 Fisiología Animal Circadian Rhythm Cryptochromes cavefish Mutation lcsh:Q sense organs Evolution of the circadian clock |
| Zdroj: | Scientific reports, 8, Art.Nr.: 8754 Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018) |
| ISSN: | 2045-2322 |
| Popis: | Light represents the principal signal driving circadian clock entrainment. However, how light influences the evolution of the clock remains poorly understood. The cavefish Phreatichthys andruzzii represents a fascinating model to explore how evolution under extreme aphotic conditions shapes the circadian clock, since in this species the clock is unresponsive to light. We have previously demonstrated that loss-of-function mutations targeting non-visual opsins contribute in part to this blind clock phenotype. Here, we have compared orthologs of two core clock genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii per2 transcript. The most abundant transcript encodes a truncated protein lacking the C-terminal Cry binding domain and incorporating an intronic, transposon-derived coding sequence. We demonstrate that the transposon insertion leads to a predominantly cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems that during evolution in complete darkness, the photic entrainment pathway of the circadian clock has been subject to mutation at multiple levels, extending from opsin photoreceptors to nuclear effectors. Universidad de Ferrara | Ref. FAR2014-2017 |
| Databáze: | OpenAIRE |
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