Glutamate agonist causes irreversible degeneration of inner hair cells
| Autor: | Shoichirou Takeda, Yoshinori Omotehara, Kiyofumi Gyo, Jun Hyodo, Nobuhiro Hakuba, Naohito Hato, Masahiro Okada |
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| Rok vydání: | 2009 |
| Předmět: |
Agonist
medicine.medical_specialty Time Factors medicine.drug_class Excitotoxicity Cell Count AMPA receptor Biology medicine.disease_cause Gerbil Internal medicine Evoked Potentials Auditory Brain Stem Excitatory Amino Acid Agonists otorhinolaryngologic diseases medicine Animals Inner ear alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Cochlea Hair Cells Auditory Inner Cell Death Dose-Response Relationship Drug General Neuroscience Glutamate receptor Neurotoxicity Neurodegenerative Diseases Dendrites Scala Tympani medicine.disease Endocrinology medicine.anatomical_structure Vacuoles Female sense organs Gerbillinae Neuroscience |
| Zdroj: | NeuroReport. 20:1255-1259 |
| ISSN: | 0959-4965 |
| DOI: | 10.1097/wnr.0b013e32833017ce |
| Popis: | Glutamate neurotoxicity in cochlear hair cells was investigated by administering the glutamate agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) into the scala tympani of Mongolian gerbils. AMPA administration caused the formation of large number of vacuoles in the inner hair cells (IHCs) and dendritic terminals. The number of degenerated hair cells was counted using rhodamine-phalloidin and Hoechst 33342 staining. The administration of 50 microM AMPA caused reversible elevation of the auditory brainstem response threshold without loss of IHCs. In contrast, 200 microM AMPA induced a substantial elevation of the auditory brainstem response threshold with the characteristic disappearance of IHCs. As cochlear ischemia involves excessive glutamate release, these results suggest that an elevated glutamate level in the cochlea is responsible for the progressive IHC death related to ischemic injury. |
| Databáze: | OpenAIRE |
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