Rat Liver-Targeted Naked Plasmid DNA Transfer by Tail Vein Injection
| Autor: | Hiroki Maruyama, Jun-ichi Miyazaki, Shigemi Kameda, Fumitake Gejyo, Noboru Higuchi |
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| Rok vydání: | 2004 |
| Předmět: |
Tail
Bioengineering Biology Applied Microbiology and Biotechnology Biochemistry Specimen Handling chemistry.chemical_compound Plasmid Gene expression medicine Animals Rats Wistar Molecular Biology Expression vector Genetic transfer Gene Transfer Techniques Transfection Molecular biology Rats medicine.anatomical_structure Liver chemistry Erythropoietin Hepatocyte Gene Targeting Injections Intravenous Algorithms DNA Plasmids Biotechnology medicine.drug |
| Zdroj: | Molecular Biotechnology. 26:165-172 |
| ISSN: | 1073-6085 |
| Popis: | High levels of foreign gene expression in mouse hepatocytes can be achieved by "hydrodynamics-based transfection," the rapid injection of a large volume of a naked deoxyribonucleic acid (DNA) solution into the tail vein. Rats are more tolerant of the frequent phlebotomies required for monitoring blood parameters than mice and, thus, are more suitable for some biomedical research. Recently, we demonstrated that hydrodynamics-based transfection can also be used to deliver naked plasmid DNA into the normal rat, which is more than 10 times larger than the mouse. We performed the tail vein injection using a syringe with a winged needle equipped with an external tube. Injection of a lac Z expression plasmid, pCAGGS-lac Z by this technique resulted in the exclusive detection of beta-galactosidase in the liver. We also injected a rat erythropoietin (Epo) expression plasmid, pCAGGS-Epo (800 microg). Maximal Epo gene expression was achieved when a 25-mL injection volume (approx 100 mL/kg body wt) was transferred within 15 s. |
| Databáze: | OpenAIRE |
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