| Popis: |
Background and Objectives: Diabetes, a lifelong progressive disease, with metabolic dysregulation, is associated with increased risk of cardiovascular complications. Paraoxonase (PON), an enzyme associated with high-density lipoprotein (HDL) is known to modulate the antioxidant and anti-inflammatory role of HDL and may have a protective role in the prevention of these complications. The present study was undertaken to evaluate serum PON, thiobarbituric acid reacting substances (TBARS) and serum lipid profile in Type 2 diabetes mellitus (DM). Materials and Methods: The study group consisted of 60 cases of clinically diagnosed Type 2 DM and 60 age and sex-matched controls. Lipid profile was estimated by enzymatic methods using EM-200 auto- analyzer, TBARS by thiobarbituric acid assay and serum PON by spectrophotometric method. Results: Type 2 DM patients showed significant increase in total cholesterol (P < 0.05), triacylglycerol (P < 0.0001), very low density lipoproteins (P < 0.0001), TBARS (P < 0.0001) levels, and significant decrease in HDL (P < 0.05) compared to controls. A significant decrease was observed in both basal (P < 0.0001) and salt stimulated PON activity in cases (P < 0.0001). Salt stimulated PON activity showed a positive correlation with HDL levels (P < 0.05) in cases, whereas basal PON activity showed negative correlation with TBARS in controls (P < 0.005). Conclusion: Type 2 DM patients have significantly lower levels of PON activity along with dyslipidemia with a direct correlation of PON with HDL levels. This may reduce the protective role of HDL and increases the susceptibility of cardiovascular complications. |
