Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as a paradigm

Autor:	S. Hanjagi, Rohini Sreevathsa, V. R. Sashidhar, Prashantkumar, Sushma Muragendra Awaji, V. Nagaveni, D. N. Madhvi
Rok vydání:	2013
Předmět:	Cadmium food.ingredient biology Physiology Abiotic stress Plant physiology chemistry.chemical_element Plant Science biology.organism_classification Plant level food chemistry Germination Seedling Botany Bioassay Agar Molecular Biology Research Article
Zdroj:	Physiology and molecular biology of plants : an international journal of functional plant biology. 16(2)
ISSN:	0971-5894
Popis:	Rigorous and stringent screening methodologies to select transformants at both seedling and plant level under cadmium or NaCl stress were developed. At seedling level, two screening strategies were standardized. One involved germination on filter paper/agar in the presence of either CdCl2 (125 μM) or NaCl (350–450 mM) for 9 days and selection of tolerant putative transformants. The other involved germination of the seedlings on soilrite by irrigation of 450 mM NaCl. Further, at plant level, in vitro evaluation for stress tolerance involved a simple leaf senescence bioassay. Combination of the seedling and plant level screening strategies would result in the initial identification of promising transformants for further analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a7eb7a1f834e615d78c079652c253d4 https://pubmed.ncbi.nlm.nih.gov/23572961 Zobrazit plný text záznamu Full text from SpringerLink