Multiple enhancer-like sequences in the HLA-B7 gene
| Autor: | Haren A. Vasavada, Subinay Ganguly, Sherman M. Weissman |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Molecular Sequence Data Restriction Mapping Genes MHC Class I Enhancer RNAs Biology Transfection Chloramphenicol acetyltransferase HLA-B7 Antigen Mice L Cells Gene expression Animals Humans Enhancer trap Enhancer Gene Multidisciplinary Base Sequence MHC Class I Gene Promoter beta-Galactosidase Molecular biology Enhancer Elements Genetic Genes HLA-B Antigens Research Article HeLa Cells Plasmids |
| Zdroj: | Proceedings of the National Academy of Sciences. 86:5247-5251 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.86.14.5247 |
| Popis: | Enhancer-like sequences have previously been identified in the promoter region of the mouse major histocompatibility complex (MHC) class I genes. We have screened for such sequences in and around a human MHC class I gene, HLA-B7. Various restriction fragments of the B7 gene were assayed for their ability to enhance transcription of a bacterial chloramphenicol acetyltransferase gene from a simian virus 40 promoter in transiently transfected mouse LTA cells. Our results demonstrate that enhancer activity is located in introns 3 and 5 as well as 5' to the transcription initiation site. RNase protection experiments corroborate the results. Preliminary experiments indicate that B7 enhancers are active in various cell types. The role of these enhancers in B7 gene expression is not known at present. We speculate that the position of the enhancer elements may be related to the occurrence of Hpa II tiny fragment islands. |
| Databáze: | OpenAIRE |
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