Evaluation of six decontamination procedures for isolation of Mycobacterium avium complex from avian feces

Autor: Zunita Zakaria, Saleha Abdul Aziz, Abdul Sattar, Rojas-Ponce Gabriel, Jalila Abu
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Nalidixic acid
Sanitization
Antibiotics
Antitubercular Agents
lcsh:Medicine
Artificial Gene Amplification and Extension
Pathology and Laboratory Medicine
Polymerase Chain Reaction
Feces
Medicine and Health Sciences
Public and Occupational Health
lcsh:Science
Decontamination
Multidisciplinary
biology
Chemistry
Antimicrobials
Eukaryota
Drugs
Human decontamination
Contamination
Mycobacterium avium Complex
Bacterial Pathogens
Actinobacteria
Laboratory Equipment
Separation Processes
Infectious Diseases
Medical Microbiology
Vertebrates
Engineering and Technology
Biological Cultures
Pathogens
medicine.drug
Mycobacterium terrae
Research Article
Infectious Disease Control
medicine.drug_class
030106 microbiology
Equipment
Research and Analysis Methods
Microbiology
Birds
03 medical and health sciences
Microbial Control
medicine
Animals
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Mycobacterium avium-intracellulare Infection
Distillation
Pharmacology
Chromatography
Bacteria
lcsh:R
Organisms
Biology and Life Sciences
Mycobacteria
biology.organism_classification
Culture Media
Health Care
030104 developmental biology
Amniotes
Nontuberculous mycobacteria
lcsh:Q
Preventive Medicine
Mycobacterium
Zdroj: PLoS ONE, Vol 13, Iss 8, p e0202034 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Culture is considered the gold standard for definitive diagnosis of mycobacterial infections. However, consensus about the most suitable culture procedure for isolation of nontuberculous mycobacteria is lacking. The study compared the recoveries of mycobacteria after decontamination of spiked and fresh avian feces with 4% sodium hydroxide (NaOH), 12% sulfuric acid (H2SO4), or 1% cetylperidinium chloride (CPC), with and without mixture of three antibiotics, namely vancomycin (VAN, 100 μg/ml), nalidixic acid (NAL, 100 μg/ml), and amphotericin B (AMB, 100 μg/ml). The antibiotic mixture was referred to as VNA. Decontamination procedures were evaluated using two (n = 2) avian fecal samples spiked with 106, 104, and 102 CFU/ml of Mycobacterium avium subsp. avium (ATCC 15769) and fresh avian feces (n = 42). M. avium subsp. avium was detected on the culture media from spiked samples (106 and 104 CFU/ml) decontaminated with NaOH, NaOH-VNA, H2SO4, and H2SO4 -VNA for 2−6 weeks. These bacteria were detected in 2–4 weeks when using CPC and CPC-VNA. M. avium subsp. avium cannot be isolated on culture media from spiked samples (102 CFU/ml) decontaminated with any decontaminating agent. Two mycobacterial isolates, namely, Mycobacterium terrae and M. engbaekii, were isolated from field samples decontaminated with NaOH and CPC-VNA. With regard to the contamination rate, the use of CPC-VNA showed lower contamination rates (5.5% and 19.0%) from spiked and field samples than those of the other methods (NaOH: 22.2% and 59.5%, NaOH-VNA: 16.7% and 21.4%, H2SO4: 11.1% and 40.5%, H2SO4-VNA: 5.5% and 21.4%, and CPC: 66.7% and 50%). In conclusion, the decontamination of fecal samples following a two-step procedure with 1% CPC and VNA can ensure high recovery rate of many mycobacteria with the lowest contamination in cultures.
Databáze: OpenAIRE
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