Stress integrated tests and cytological analyses reveal Brassica villosa subsp. drepanensis seed quality decrease upon long-term storage
| Autor: | Anna Scialabba, Lorenza Bellani, Lucia Giorgetti |
|---|---|
| Přispěvatelé: | Scialabba, A, Giorgetti, L, Bellani, LM |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitotic index Plant Science Biology genebank Brassica villosa subsp. drepanensis genebank genotoxicity germination seed storage stress integrated germination test 01 natural sciences Brassica villosa subsp. drepanensis genotoxicity germination seed storage stress integrated germination test Settore BIO/01 - Botanica Generale 03 medical and health sciences Osmotic pressure Ecology Evolution Behavior and Systematics food and beverages Horticulture Brassica villosa 030104 developmental biology Agronomy Germination 010606 plant biology & botany |
| Zdroj: | Plant Biosystems (Firenze, Online) (2014). doi:10.1080/11263504.2014.991359 info:cnr-pdr/source/autori:A. Scialabba, L. Giorgetti, L.M. Bellani/titolo:Stress integrated tests and cytological analyses reveal Brassica villosa subsp. drepanensis seed quality decrease upon long-term storage/doi:10.1080%2F11263504.2014.991359/rivista:Plant Biosystems (Firenze, Online)/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| DOI: | 10.1080/11263504.2014.991359 |
| Popis: | Under stress integrated germination test (SIGT), seeds undergo osmo-saline stresses, which enable to detect differences in vigour of long-term stored seeds with high germination percentage (G%). The quality of Brassica villosa subsp. drepanensis seeds stored in a genebank (at -20°C for 16 years) was compared with seeds at harvest by standard germination tests (GT), SIGT and cytogenetic analysis. No differences were detected in G% and mean germination time under GT. Conversely, SIGT performed with NaCl -0.9MPa osmotic potential did not influence G% at harvest but reduced that of stored seeds, SIGT at -1.4MPa reduced G% of both. Cytogenetic analysis showed reduction of mitotic index, appearance of chromosomal aberrations and smaller nucleoli in stored seeds compared with harvest seeds germinated in water. SIGT at -0.9MPa had no effect on mitotic index, but increased chromosome aberrations and nucleoli number. SIGT at -1.4MPa inhibited G% of harvest and stored seeds, reduced mitoses in harvest and completely prevented it in stored seeds. The results indicate that GT does not faithfully reflect the quality of stored seeds, with misinterpretation of their vigour, whereas SIGT and cytogenetical parameters are sensitive, reliable and inexpensive methods for early prediction of genetic erosion in germplasm banks. |
| Databáze: | OpenAIRE |
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