BMP-2 but not VEGF or PDGF in fibrin matrix supports bone healing in a delayed-union rat model

Autor: Arthur Schultz, Martijn van Griensven, Sebastian Schützenberger, Paul Slezak, James Ferguson, Tatjana J. Morton, Heinz Redl, Martin Kaipel
Rok vydání: 2012
Předmět:
Vascular Endothelial Growth Factor A
Male
Platelet-Derived Growth Factor/pharmacology
medicine.medical_specialty
VEGF receptors
Arthrodesis
medicine.medical_treatment
Rat model
Fibrin matrix
Bone Morphogenetic Protein 2
Bone healing
Ununited/drug therapy
Vascular Endothelial Growth Factor A/pharmacology
Bone morphogenetic protein 2
Fibrin
Rats
Sprague-Dawley
Animals
Medicine
Orthopedics and Sports Medicine
Femur
Recombinant Proteins/pharmacology
Bone Morphogenetic Protein 2/pharmacology
Fracture Healing
Platelet-Derived Growth Factor
Femoral Fractures/drug therapy
biology
business.industry
Fracture Healing/drug effects
Femur/pathology
Recombinant Proteins
Rats
Surgery
Fractures
Ununited
biology.protein
Sprague-Dawley
Fibrin/pharmacokinetics
business
Femoral Fractures
Fractures
Platelet-derived growth factor receptor
Zdroj: Journal of Orthopaedic Research. 30:1563-1569
ISSN: 0736-0266
DOI: 10.1002/jor.22132
Popis: Treatment of delayed bone healing and non-unions after fractures, osteotomies or arthrodesis still is a relevant clinical challenge. Artificially applied growth factors can increase bone healing and progressively gain importance in clinical routine. The aim of this study was to determine the effects of rhPDGF-BB, rhVEGF-165, and rhBMP-2 in fibrin matrix on bone healing in a delayed-union rat model. Thirty-seven rats underwent a first operation where a standardized femoral critical size defect was created. A silicone spacer was implanted to impair vascularization within the defect. At 4 weeks the spacer was removed in a second operation and rhPDGF-BB, rhVEGF-165, or rhBMP-2 were applied in a fibrin clot. Animals in a fourth group received a fibrin clot without growth factors. At 8 weeks fibrin bound rhBMP-2 treated animals showed a significantly increased union rate and bone volume within the defect compared to the other groups. Single application of fibrin bound rhPDGF-BB and rhVEGF-165 failed to increase bone healing in our atrophic non-union model. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1563–1569, 2012
Databáze: OpenAIRE
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