Day-Night Oscillation of Atrogin1 and Timing-Dependent Preventive Effect of Weight-Bearing on Muscle Atrophy
| Autor: | Natsumi Aoki, Yuta Hattori, Kengo Takahashi, Ryosuke Ishikawa, Shigenobu Shibata, Rina Hirooka, Atsushi Haraguchi, Mizuho Tanaka, Keisuke Sasaki, Shuichi Kojima, Shinya Aoyama, Miku Takizawa, Takeru Shimoda |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Research paper Mutant Circadian clock Muscle Proteins Context (language use) Biology medicine.disease_cause General Biochemistry Genetics and Molecular Biology Weight-bearing Weight-Bearing 03 medical and health sciences Mice Internal medicine Physical Conditioning Animal medicine Animals Circadian rhythm Mice Inbred ICR SKP Cullin F-Box Protein Ligases Therapeutic effect General Medicine Muscle atrophy Mice Mutant Strains Muscular Atrophy 030104 developmental biology Endocrinology Chrono-exercise Gene Expression Regulation Hindlimb-unloading Sciatic nerve medicine.symptom Atrogin1 |
| Zdroj: | EBioMedicine |
| ISSN: | 2352-3964 |
| Popis: | Background Atrogin1, which is one of the key genes for the promotion of muscle atrophy, exhibits day-night variation. However, its mechanism and the role of its day-night variation are largely unknown in a muscle atrophic context. Methods The mice were induced a muscle atrophy by hindlimb-unloading (HU). To examine a role of circadian clock, Wild-type (WT) and Clock mutant mice were used. To test the effects of a neuronal effects, an unilateral ablation of sciatic nerve was performed in HU mice. To test a timing-dependent effects of weight-bearing, mice were released from HU for 4 h in a day at early or late active phase (W-EAP and W-LAP groups, respectively). Findings We found that the day-night oscillation of Atrogin1 expression was not observed in Clock mutant mice or in the sciatic denervated muscle. In addition, the therapeutic effects of weight-bearing were dependent on its timing with a better effect in the early active phase. Interpretation These findings suggest that the circadian clock controls the day-night oscillation of Atrogin1 expression and the therapeutic effects of weight-bearing are dependent on its timing. Fund Council for Science, Technology, and Innovation, SIP, “Technologies for creating next-generation agriculture, forestry, and fisheries”. Highlights • Atrogin1 expression shows day-night variation in a muscle atrophic context. • Preventive effects of intermittent weight-bearing on muscle loss are dependent on its timing. |
| Databáze: | OpenAIRE |
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