Distinct Cyclin D Genes Show Mitotic Accumulation or Constant Levels of Transcripts in Tobacco Bright Yellow-2 Cells1
| Autor: | James A. H. Murray, Nicole Chaubet-Gigot, Claude Gigot, Bruno Combettes, David A. Sorrell |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Physiology
Nicotiana tabacum Cyclin D Cyclin A Mitosis Plant Science Genes Plant Gene Expression Regulation Plant Cyclins Tobacco Genetics Animals Cyclin Plant Proteins biology G1 Phase Cell cycle Plant cell biology.organism_classification Cell biology Plants Toxic Cell culture RNA Plant biology.protein Research Article |
| Popis: | The commitment of eukaryotic cells to division normally occurs during the G1 phase of the cell cycle. In mammals D-type cyclins regulate the progression of cells through G1 and therefore are important for both proliferative and developmental controls. Plant CycDs (D-type cyclin homologs) have been identified, but their precise function during the plant cell cycle is unknown. We have isolated three tobacco (Nicotiana tabacum) CycD cyclin cDNAs: two belong to the CycD3 class (Nicta;CycD3;1 and Nicta;CycD3;2) and the third to the CycD2 class (Nicta;CycD2;1). To uncouple their cell-cycle regulation from developmental control, we have used the highly synchronizable tobacco cultivar Bright Yellow-2 in a cell-suspension culture to characterize changes in CycD transcript levels during the cell cycle. In cells re-entering the cell cycle from stationary phase, CycD3;2 was induced in G1 but subsequently remained at a constant level in synchronous cells. This expression pattern is consistent with a role forCycD3;2, similar to mammalian D-type cyclins. In contrast, CycD2;1 and CycD3;1 transcripts accumulated during mitosis in synchronous cells, a pattern of expression not normally associated with D-type cyclins. This could suggest a novel role for plant D-type cyclins during mitosis. |
| Databáze: | OpenAIRE |
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