Association testing by DNA pooling: An effective initial screen
| Autor: | Gail Isabel Reid Adam, Andi Braun, Patrick W. Kleyn, Stefan Kammerer, Charles R. Cantor, Dirk van den Boom, Christiane Honisch, Aruna Bansal |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
Adolescent Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Cholesterylester transfer protein Humans Allele Gene Genotyping Alleles Aged Glycoproteins Genetic association Genetics Multidisciplinary Cholesterol HDL Haplotype Gene Pool Biological Sciences Middle Aged Cholesterol Ester Transfer Proteins SNP genotyping Haplotypes biology.protein Female Carrier Proteins |
| Zdroj: | Proceedings of the National Academy of Sciences. 99:16871-16874 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | With an ever-increasing resource of validated single-nucleotide polymorphisms (SNPs), the limiting factors in genome-wide association analysis have become genotyping capacity and the availability of DNA. We provide a proof of concept of the use of pooled DNA as a means of efficiently screening SNPs and prioritizing them for further study. This approach reduces the final number of SNPs that undergo full, sample-by-sample genotyping as well as the quantity of DNA used overall. We have examined 15 SNPs in the cholesteryl ester transfer protein ( CETP ) gene, a gene previously demonstrated to be associated with serum high-density lipoprotein cholesterol levels. The SNPs were amplified in two pools of DNA derived from groups of individuals with extremely high and extremely low serum high-density lipoprotein cholesterol levels, respectively. P values |
| Databáze: | OpenAIRE |
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