Transcriptome Analysis for Abnormal Spike Development of the Wheat Mutant dms
| Autor: | Dong Yan Yu, Chun Cai Shen, Qiaoyun Li, Yu Mei Jiang, Zong Biao Duan, Xinxin Zhu, Ji Shan Niu, Yong Jing Ni |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Metabolic Processes UniGene lcsh:Medicine Gene Expression 01 natural sciences Biochemistry Transcriptome lcsh:Science Triticum Genetics Multidisciplinary food and beverages Agriculture Plants Phenotype Wheat Carbohydrate Metabolism Metabolic Pathways Homeotic gene Research Article Carbohydrate transport Locus (genetics) Crops Biology Genes Plant 03 medical and health sciences DNA-binding proteins Gene Regulation RNA Messenger Grasses Gene Gene Expression Profiling lcsh:R Organisms Biology and Life Sciences Proteins Regulatory Proteins Gene expression profiling 030104 developmental biology Metabolism Mutation lcsh:Q 010606 plant biology & botany Crop Science Cereal Crops Transcription Factors |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 3, p e0149287 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Background Wheat (Triticum aestivum L.) spike development is the foundation for grain yield. We obtained a novel wheat mutant, dms, characterized as dwarf, multi-pistil and sterility. Although the genetic changes are not clear, the heredity of traits suggests that a recessive gene locus controls the two traits of multi-pistil and sterility in self-pollinating populations of the medium plants (M), such that the dwarf genotype (D) and tall genotype (T) in the progeny of the mutant are ideal lines for studies regarding wheat spike development. The objective of this study was to explore the molecular basis for spike abnormalities of dwarf genotype. Results Four unigene libraries were assembled by sequencing the mRNAs of the super-bulked differentiating spikes and stem tips of the D and T plants. Using integrative analysis, we identified 419 genes highly expressed in spikes, including nine typical homeotic genes of the MADS-box family and the genes TaAP2, TaFL and TaDL. We also identified 143 genes that were significantly different between young spikes of T and D, and 26 genes that were putatively involved in spike differentiation. The result showed that the expression levels of TaAP1-2, TaAP2, and other genes involved in the majority of biological processes such as transcription, translation, cell division, photosynthesis, carbohydrate transport and metabolism, and energy production and conversion were significantly lower in D than in T. Conclusions We identified a set of genes related to wheat floral organ differentiation, including typical homeotic genes. Our results showed that the major causal factors resulting in the spike abnormalities of dms were the lower expression homeotic genes, hormonal imbalance, repressed biological processes, and deficiency of construction materials and energy. We performed a series of studies on the homeotic genes, however the other three causal factors for spike abnormal phenotype of dms need further study. |
| Databáze: | OpenAIRE |
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