Phylogeny of the Mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene
| Autor: | Armelle David, Sophie Lorenzon, François Thiaucourt, Alain Bréard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: |
DNA
Bacterial Phylogénie Sequence analysis Analyse biologique Molecular Sequence Data Cattle Diseases Locus (genetics) Sequence alignment Microbiology Polymerase Chain Reaction DNA sequencing Contagious caprine pleuropneumonia Contagious bovine pleuropneumonia medicine Animals Cluster Analysis L50 - Physiologie et biochimie animales Insertion sequence Cloning Molecular Pleuropneumonia Contagious Phylogeny DNA Primers Gene Library Genetics Goat Diseases General Veterinary biology Base Sequence Goats Mycoplasma mycoides General Medicine Sequence Analysis DNA biology.organism_classification medicine.disease PCR Gène Cattle Sequence Alignment Bacterial Outer Membrane Proteins |
| Zdroj: | Veterinary Microbiology |
| Popis: | The Mycoplasma mycoides cluster is made of six species that are closely related both genetically and phenotypically. Two are of particular importance, M. mycoides subsp, mycoides SC causing contagious bovine pleuropneumonia and M. capricolum subsp, capripneumoniae causing contagious caprine pleuropneumonia. The sequences of a putative membrane protein gene and partial flanking open reading frames have been obtained from various strains in this cluster, including all reference strains. Sequence analysis showed this locus is present and fully conserved in all strains of M. mycoides subsp, mycoides SC isolated from geographically most distant places worldwide. In M. capricolum subsp. capripneumoniae polymorphism in this locus has been found at seven positions and revealed that they can be used as epidemiological markers. Conserved regions were used to define a primer pair that enables the amplification by PCR of two fragments 302 and 1298 bp long, respectively. The 302 bp long fragment contains an intergenic sequence that can be used for phylogenetic studies or for identification purposes. Parsimony analysis on an alignment of 49 DNA sequences show a subdivision of the M. mycoides cluster into two subgroups that is in accordance with results obtained by phenotypic methods. Two lineages exist within the capricolum subgroup, one of them clustering strains identified as M. capricolum subsp. capricolum, M. capricolum subsp. capricolum and M. sp Bovine Group 7. However M. capricolum subsp. capripneumoniae strains can readily be identified by three specific nucleotide positions or by sequencing the 1298 bp long fragment. There is no clear subdivision within the mycoides subgroup, supporting the idea that M. mycoides subsp. mycoides LC and M. mycoides subsp. capri should not be separated into two subspecies. Mycoplasma mycoides subsp. mycoides SC strains can easily be distinguished as they bear an insertion sequence 15 bp downstream from the stop codon of the membrane protein gene. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|